V Ignjatovic1, E Straka, R Summerhayes, P Monagle. 1. Murdoch Childrens Research Institute, The University of Melbourne, Royal Childrens Hospital, Parkville, Victoria, Australia. verai@unimelb.edu.au
Abstract
BACKGROUND: Clinically significant age-related differences in the anticoagulation effect of heparin have previously been established in vitro as well as in different clinical settings in vivo. These differences were hypothesized to be due to the age-specific differences in binding of heparin to plasma proteins. OBJECTIVES: The aim of this project was to investigate global age-related differences in heparin binding to plasma proteins. PATIENTS/ METHODS: Heparin-binding proteins were identified by incubating heparin-coated magnetic beads with plasma samples from neonates, children and adults, and purifying the proteins that were bound to the beads in this reaction system. RESULTS: These results provide the first preliminary evidence of age-related differences in the total number and concentration of proteins bound to heparin. The results also suggest, for the first time, that there are age-related differences of heparin binding to antithrombin and thrombin. CONCLUSIONS: The results of this study, although preliminary, support and contribute to the explanation of the mechanism of age-related differences in the effect of heparin observed previously in vitro and in vivo.
BACKGROUND: Clinically significant age-related differences in the anticoagulation effect of heparin have previously been established in vitro as well as in different clinical settings in vivo. These differences were hypothesized to be due to the age-specific differences in binding of heparin to plasma proteins. OBJECTIVES: The aim of this project was to investigate global age-related differences in heparin binding to plasma proteins. PATIENTS/ METHODS:Heparin-binding proteins were identified by incubating heparin-coated magnetic beads with plasma samples from neonates, children and adults, and purifying the proteins that were bound to the beads in this reaction system. RESULTS: These results provide the first preliminary evidence of age-related differences in the total number and concentration of proteins bound to heparin. The results also suggest, for the first time, that there are age-related differences of heparin binding to antithrombin and thrombin. CONCLUSIONS: The results of this study, although preliminary, support and contribute to the explanation of the mechanism of age-related differences in the effect of heparin observed previously in vitro and in vivo.
Authors: Paul Monagle; Anthony K C Chan; Neil A Goldenberg; Rebecca N Ichord; Janna M Journeycake; Ulrike Nowak-Göttl; Sara K Vesely Journal: Chest Date: 2012-02 Impact factor: 9.410
Authors: Stefan Bjelosevic; Dana Pascovici; Hui Ping; Vasiliki Karlaftis; Thiri Zaw; Xiaomin Song; Mark P Molloy; Paul Monagle; Vera Ignjatovic Journal: Mol Cell Proteomics Date: 2017-03-23 Impact factor: 5.911
Authors: Devendra Saksena; Yugal K Mishra; S Muralidharan; Vivek Kanhere; Pankaj Srivastava; C P Srivastava Journal: Indian J Thorac Cardiovasc Surg Date: 2019-01-28
Authors: Vera Ignjatovic; Cera Lai; Robyn Summerhayes; Ulrike Mathesius; Sherif Tawfilis; Matthew A Perugini; Paul Monagle Journal: PLoS One Date: 2011-02-18 Impact factor: 3.240
Authors: Hui Ping Yaw; Suelyn Van Den Helm; Graeme MacLaren; Matthew Linden; Paul Monagle; Vera Ignjatovic Journal: Front Cardiovasc Med Date: 2019-09-18