| Literature DB >> 20217615 |
Adam J Kimple1, Robin E Muller, David P Siderovski, Francis S Willard.
Abstract
Surface plasmon resonance (SPR) is a robust method to detect and quantify macromolecular interactions; however, to measure binding interactions, one component must be immobilized on a sensor surface. This is typically achieved using covalent immobilization via free amines or thiols, or noncovalent immobilization using high-affinity interactions such as biotin/streptavidin or antibody/antigen. In this chapter we describe a robust method to covalently immobilize His(6) fusion proteins on the sensor surface for SPR analysis.Entities:
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Year: 2010 PMID： 20217615 PMCID： PMC3031178 DOI： 10.1007/978-1-60761-670-2_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745