INTRODUCTION: Animal models have been used as insight into the pathogenesis of osteonecrosis, even though most have failed to reproduce all stages of human disease, limiting progression in experimental treatment modalities. A new surgically induced animal model of femoral head osteonecrosis in sheep is presented. METHOD: Osteonecrosis was achieved using an improved method of intracephalic cryogenic lesion by means of a cryoprobe and vascular ligation. RESULTS: Histologic findings at 6 and 12 weeks showed progression to advanced stage osteonecrosis. MRI, the gold standard in diagnosis and follow-up in human osteonecrosis showed concordant results with histology. CONCLUSION: Our model can therefore be used as a preclinical femoral head osteonecrosis model in an easily accessible animal to assess biological treatments with MRI.
INTRODUCTION: Animal models have been used as insight into the pathogenesis of osteonecrosis, even though most have failed to reproduce all stages of human disease, limiting progression in experimental treatment modalities. A new surgically induced animal model of femoral head osteonecrosis in sheep is presented. METHOD:Osteonecrosis was achieved using an improved method of intracephalic cryogenic lesion by means of a cryoprobe and vascular ligation. RESULTS: Histologic findings at 6 and 12 weeks showed progression to advanced stage osteonecrosis. MRI, the gold standard in diagnosis and follow-up in humanosteonecrosis showed concordant results with histology. CONCLUSION: Our model can therefore be used as a preclinical femoral head osteonecrosis model in an easily accessible animal to assess biological treatments with MRI.
Authors: Nobuhiro Kamiya; Ryosuke Yamaguchi; Olumide Aruwajoye; Naga Suresh Adapala; Harry K W Kim Journal: Clin Orthop Relat Res Date: 2015-02-10 Impact factor: 4.176
Authors: Reinhard Meier; Tobias M Kraus; Christoph Schaeffeler; Sebastian Torka; Anna Melissa Schlitter; Katja Specht; Bernhard Haller; Simone Waldt; Hans Rechl; Ernst J Rummeny; Klaus Woertler Journal: Eur Radiol Date: 2014-05-28 Impact factor: 5.315
Authors: B Salmon; B Liu; E Shen; T Chen; J Li; M Gillette; R C Ransom; M Ezran; C A Johnson; A B Castillo; W J Shen; F B Kraemer; A A Smith; J A Helms Journal: Sci Rep Date: 2017-10-27 Impact factor: 4.379