METHOD: We injected two drugs that modify the epigenome, the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine (5-aza-dC) and the histone deacetylase inhibitor trichostatin A (TSA), alone or in combination, into C57Bl/6 mice subjected to amputation through the mid-second phalanx of the third digit. Wound-site tissue was collected. RESULTS: We observed increased staining of the stem cell markers Rex1 (Zfp42) and stem cell antigen-1 at digit amputation sites from drug-treated mice. Samples from 5-aza-dC plus TSA and TSA treated mice also showed increased proliferating cell nuclear antigen staining, a measure of cell proliferation. Drug treatments increased Msx1, but not Cyp26a1 or ALDH1a2 (RALDH2) mRNA. CONCLUSION: 5-aza-dC and TSA treatments stimulated cell proliferation at the amputation site, possibly via increased expression of genes involved in digit development and regeneration.
METHOD: We injected two drugs that modify the epigenome, the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine (5-aza-dC) and the histone deacetylase inhibitor trichostatin A (TSA), alone or in combination, into C57Bl/6 mice subjected to amputation through the mid-second phalanx of the third digit. Wound-site tissue was collected. RESULTS: We observed increased staining of the stem cell markers Rex1 (Zfp42) and stem cell antigen-1 at digit amputation sites from drug-treated mice. Samples from 5-aza-dC plus TSA and TSA treated mice also showed increased proliferating cell nuclear antigen staining, a measure of cell proliferation. Drug treatments increased Msx1, but not Cyp26a1 or ALDH1a2 (RALDH2) mRNA. CONCLUSION:5-aza-dC and TSA treatments stimulated cell proliferation at the amputation site, possibly via increased expression of genes involved in digit development and regeneration.
Authors: Chiara Cianciolo Cosentino; Nataliya I Skrypnyk; Lauren L Brilli; Takuto Chiba; Tatiana Novitskaya; Clara Woods; James West; Vasiliy N Korotchenko; Lee McDermott; Billy W Day; Alan J Davidson; Raymond C Harris; Mark P de Caestecker; Neil A Hukriede Journal: J Am Soc Nephrol Date: 2013-04-25 Impact factor: 10.121
Authors: Warnakulasuriya Akash Fernando; Eric Leininger; Jennifer Simkin; Ni Li; Carrie A Malcom; Shyam Sathyamoorthi; Manjong Han; Ken Muneoka Journal: Dev Biol Date: 2010-12-08 Impact factor: 3.582
Authors: Christopher J Lewis; Andrei N Mardaryev; Andrey A Sharov; Michael Y Fessing; Vladimir A Botchkarev Journal: Adv Wound Care (New Rochelle) Date: 2014-07-01 Impact factor: 4.730