BACKGROUND: In this study, we investigated the biochemical effectiveness of methylprednisolone, N-acetylcysteine (NAC) and methylprednisolone combined with NAC treatment in experimental spinal cord injury in rats. METHODS: Thirty-two Sprague-Dawley male rats weighing 250-300 g were divided into four groups. Spinal cord injury was created extradurally with an aneurysm clip at the T4-T5 level. Following the trauma, Group C (Control group, n:8) was not given any treatment. Group M (methylprednisolone group, n:8) was treated with 30 mg x kg(-1) methylprednisolone followed by a maintenance dose of 5.4 mg x kg(-1) per hour. Group N (NAC group, n:8) was given 150 mg x kg(-1) NAC. Group MN (methylprednisolone and NAC group, n:8) was given 30 mg x kg(-1) followed by an hourly maintenance dose of 5.4 mg x kg(-1) methylprednisolone and 150 mg x kg(-1) NAC intraperitoneally. Twenty-four hours after the trauma, the rats were decapitated under anesthesia, and their spinal cord samples were taken for biochemical examination. RESULTS: Mean malonyldialdehyde (MDA) values in Groups M, N and MN were significantly reduced compared to Group C. Mean superoxide dismutase (SOD) values in Groups M, N and MN were significantly higher than in Group C (p<0.05). No difference existed between Groups M and N with respect to mean MDA and SOD values. CONCLUSION: Methylprednisolone, NAC and methylprednisolone plus NAC treatments have potential biochemical benefits in preventing secondary injury in experimental spinal cord injury in rats.
BACKGROUND: In this study, we investigated the biochemical effectiveness of methylprednisolone, N-acetylcysteine (NAC) and methylprednisolone combined with NAC treatment in experimental spinal cord injury in rats. METHODS: Thirty-two Sprague-Dawley male rats weighing 250-300 g were divided into four groups. Spinal cord injury was created extradurally with an aneurysm clip at the T4-T5 level. Following the trauma, Group C (Control group, n:8) was not given any treatment. Group M (methylprednisolone group, n:8) was treated with 30 mg x kg(-1) methylprednisolone followed by a maintenance dose of 5.4 mg x kg(-1) per hour. Group N (NAC group, n:8) was given 150 mg x kg(-1) NAC. Group MN (methylprednisolone and NAC group, n:8) was given 30 mg x kg(-1) followed by an hourly maintenance dose of 5.4 mg x kg(-1) methylprednisolone and 150 mg x kg(-1) NAC intraperitoneally. Twenty-four hours after the trauma, the rats were decapitated under anesthesia, and their spinal cord samples were taken for biochemical examination. RESULTS: Mean malonyldialdehyde (MDA) values in Groups M, N and MN were significantly reduced compared to Group C. Mean superoxide dismutase (SOD) values in Groups M, N and MN were significantly higher than in Group C (p<0.05). No difference existed between Groups M and N with respect to mean MDA and SOD values. CONCLUSION:Methylprednisolone, NAC and methylprednisolone plus NAC treatments have potential biochemical benefits in preventing secondary injury in experimental spinal cord injury in rats.
Authors: Ramon Diaz-Arrastia; Patrick M Kochanek; Peter Bergold; Kimbra Kenney; Christine E Marx; Col Jamie B Grimes; L T C Yince Loh; L T C Gina E Adam; Devon Oskvig; Kenneth C Curley; Wanda Salzer Journal: J Neurotrauma Date: 2014-01-15 Impact factor: 5.269
Authors: U Y Çavuş; A Yılmaz; M N Aytekin; G Taburcu; A Albayrak; S Yıldırım; I Ağır Journal: Eur J Trauma Emerg Surg Date: 2013-11-21 Impact factor: 3.693
Authors: Andrew N Stewart; Ethan P Glaser; Caitlin A Mott; William M Bailey; Patrick G Sullivan; Samir P Patel; John C Gensel Journal: J Neurotrauma Date: 2022-07-13 Impact factor: 4.869