Crystallization and preliminary X-ray analysis of the diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv.

A novel diadenosine 5',5'''-P(1),P(4)-tetraphosphate (Ap4A) phosphorylase (Rv2613c) from Mycobacterium tuberculosis H37Rv has been crystallized by the sitting-drop vapour-diffusion method. The crystal belonged to space group C2, with unit-cell parameters a = 101.5, b = 63.6, c = 79.1 A, beta = 110.9 degrees. The diffraction of the crystals extended to 1.9 A resolution. The asymmetric unit is expected to contain two molecules of Rv2613c, with a corresponding crystal volume per protein weight (V(M)) of 2.41 A(3) Da(-1) and a solvent content of 49.1%. This is the first report of a crystal of Ap4A phosphorylase.