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Literature DB >> 20206959

Vaccinia H5 is a multifunctional protein involved in viral DNA replication, postreplicative gene transcription, and virion morphogenesis.

Susan M D'Costa¹, Travis W Bainbridge, Sayuri E Kato, Cindy Prins, Karen Kelley, Richard C Condit.

Abstract

The vaccinia H5 protein has been implicated in several steps of virus replication including DNA synthesis, postreplicative gene transcription, and virion morphogenesis. Our recent mapping of mutants in the consolidated Condit-Dales collection identified a temperature-sensitive vaccinia mutant in the H5R gene (Dts57). We demonstrate here that Dts57 has a DNA negative phenotype, strongly suggesting a direct role for H5 in DNA replication. We used a temperature shift protocol to determine the impact of H5 temperature sensitivity on postreplicative gene expression and observed changes in the pattern of postreplicative viral mRNA metabolism consistent with a role of H5 in postreplicative transcription. Finally, using a rifampicin release temperature shift protocol, we show that H5 is involved in multiple steps of virion morphogenesis. These data demonstrate directly that H5 plays roles in DNA replication, transcription and morphogenesis in vivo.

Entities: CellLine Chemical Disease Gene Mutation Species

Mesh：

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Year: 2010 PMID： 20206959 PMCID： PMC2849846 DOI： 10.1016/j.virol.2010.01.020

Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616

45 in total

1. Genome-wide analysis of vaccinia virus protein-protein interactions.

Authors: S McCraith; T Holtzman; B Moss; S Fields
Journal: Proc Natl Acad Sci U S A Date: 2000-04-25 Impact factor: 11.205

2. Clustered charge-to-alanine mutagenesis of the vaccinia virus H5 gene: isolation of a dominant, temperature-sensitive mutant with a profound defect in morphogenesis.

Authors: J DeMasi; P Traktman
Journal: J Virol Date: 2000-03 Impact factor: 5.103

3. Role of vaccinia virus A20R protein in DNA replication: construction and characterization of temperature-sensitive mutants.

Authors: K Ishii; B Moss
Journal: J Virol Date: 2001-02 Impact factor: 5.103

4. High-frequency genetic recombination and reactivation of orthopoxviruses from DNA fragments transfected into leporipoxvirus-infected cells.

Authors: Xiao-Dan Yao; David H Evans
Journal: J Virol Date: 2003-07 Impact factor: 5.103

5. Mapping interaction sites of the A20R protein component of the vaccinia virus DNA replication complex.

Authors: Koji Ishii; Bernard Moss
Journal: Virology Date: 2002-11-25 Impact factor: 3.616

6. Post-transcription cleavage generates the 3' end of F17R transcripts in vaccinia virus.

Authors: Susan M D'Costa; James B Antczak; David J Pickup; Richard C Condit
Journal: Virology Date: 2004-02-05 Impact factor: 3.616

7. Temperature-dependent phosphorylation state of the H5R protein synthesised at the early stage of infection in cells infected with vaccinia virus ts mutants of the B1R and F10L protein kinases.

Authors: G Beaud; R Beaud
Journal: Intervirology Date: 2000 Impact factor: 1.763

8. Complementation analysis of the dales collection of vaccinia virus temperature-sensitive mutants.

Authors: Cari A Lackner; Susan M D'Costa; Charles Buck; Richard C Condit
Journal: Virology Date: 2003-01-20 Impact factor: 3.616

9. Poxviral B1 kinase overcomes barrier to autointegration factor, a host defense against virus replication.

Authors: Matthew S Wiebe; Paula Traktman
Journal: Cell Host Microbe Date: 2007-05-17 Impact factor: 21.023

Review 10. Regulation of viral transcription elongation and termination during vaccinia virus infection.

Authors: Richard C Condit; Edward G Niles
Journal: Biochim Biophys Acta Date: 2002-09-13

13 in total

1. Identification of protein-protein interaction inhibitors targeting vaccinia virus processivity factor for development of antiviral agents.

Authors: Norbert Schormann; Charnell Inglis Sommers; Mark N Prichard; Kathy A Keith; James W Noah; Manunya Nuth; Robert P Ricciardi; Debasish Chattopadhyay
Journal: Antimicrob Agents Chemother Date: 2011-08-15 Impact factor: 5.191

2. Participation of vaccinia virus l2 protein in the formation of crescent membranes and immature virions.

Authors: Liliana Maruri-Avidal; Arban Domi; Andrea S Weisberg; Bernard Moss
Journal: J Virol Date: 2011-01-12 Impact factor: 5.103

3. Evaluation of the role of the vaccinia virus uracil DNA glycosylase and A20 proteins as intrinsic components of the DNA polymerase holoenzyme.

Authors: Kathleen A Boyle; Eleni S Stanitsa; Matthew D Greseth; Jill K Lindgren; Paula Traktman
Journal: J Biol Chem Date: 2011-05-13 Impact factor: 5.157

4. Molecular genetic and biochemical characterization of the vaccinia virus I3 protein, the replicative single-stranded DNA binding protein.

Authors: Matthew D Greseth; Kathleen A Boyle; Matthew S Bluma; Bethany Unger; Matthew S Wiebe; Jamaria A Soares-Martins; Nadi T Wickramasekera; James Wahlberg; Paula Traktman
Journal: J Virol Date: 2012-03-21 Impact factor: 5.103

5. Genetic Confirmation that the H5 Protein Is Required for Vaccinia Virus DNA Replication.

Authors: Kathleen A Boyle; Matthew D Greseth; Paula Traktman
Journal: J Virol Date: 2015-04-08 Impact factor: 5.103

6. Vaccinia virus A6 is essential for virion membrane biogenesis and localization of virion membrane proteins to sites of virion assembly.

Authors: Xiangzhi Meng; Addie Embry; Lloyd Rose; Bo Yan; Chungui Xu; Yan Xiang
Journal: J Virol Date: 2012-03-07 Impact factor: 5.103

10. Vaccinia Virus B1 Kinase Is Required for Postreplicative Stages of the Viral Life Cycle in a BAF-Independent Manner in U2OS Cells.

Authors: Augusta Jamin; Nouhou Ibrahim; April Wicklund; Kaitlin Weskamp; Matthew S Wiebe
Journal: J Virol Date: 2015-07-29 Impact factor: 5.103