Literature DB >> 20206514

A reverse transcriptase stop assay revealed diverse quadruplex formations in UTRs in mRNA.

Masaki Hagihara1, Keisuke Yoneda, Hiroaki Yabuuchi, Yasushi Okuno, Kazuhiko Nakatani.   

Abstract

Here, we developed a reverse transcriptase based method (RTase stop assay) to characterize quadruplex formations in guanine-rich RNAs with high sensitivity and specificity. By using the RTase stop assay, we also revealed a plausible structural polymorphism in biologically important RNAs. The RTase stop assay would provide helpful insight into RNA quadruplex structures and functions, together with other analytical methods, including various footprinting techniques. 2010 Elsevier Ltd. All rights reserved.

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Year:  2010        PMID： 20206514     DOI： 10.1016/j.bmcl.2010.01.158

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  9 in total

1.  Macrocyclic biphenyl tetraoxazoles: synthesis, evaluation as G-quadruplex stabilizers and cytotoxic activity.

Authors:  Gifty A Blankson; Daniel S Pilch; Angela A Liu; Leroy F Liu; Joseph E Rice; Edmond J LaVoie
Journal:  Bioorg Med Chem       Date:  2013-05-30       Impact factor: 3.641

2.  Targeted Detection of G-Quadruplexes in Cellular RNAs.

Authors:  Chun Kit Kwok; Shankar Balasubramanian
Journal:  Angew Chem Int Ed Engl       Date:  2015-04-23       Impact factor: 15.336

3.  Macrocyclic pyridyl polyoxazoles: structure-activity studies of the aminoalkyl side-chain on G-quadruplex stabilization and cytotoxic activity.

Authors:  Gifty Blankson; Suzanne G Rzuczek; Cody Bishop; Daniel S Pilch; Angela Liu; Leroy Liu; Edmond J Lavoie; Joseph E Rice
Journal:  Molecules       Date:  2013-09-26       Impact factor: 4.411

4.  Multiple G-quartet structures in pre-edited mRNAs suggest evolutionary driving force for RNA editing in trypanosomes.

Authors:  W-Matthias Leeder; Niklas F C Hummel; H Ulrich Göringer
Journal:  Sci Rep       Date:  2016-07-20       Impact factor: 4.379

5.  Small molecule-based detection of non-canonical RNA G-quadruplex structures that modulate protein translation.

Authors:  Yousuke Katsuda; Shin-Ichi Sato; Maimi Inoue; Hisashi Tsugawa; Takuto Kamura; Tomoki Kida; Rio Matsumoto; Sefan Asamitsu; Norifumi Shioda; Shuhei Shiroto; Yoshiki Oosawatsu; Kenji Yatsuzuka; Yusuke Kitamura; Masaki Hagihara; Toshihiro Ihara; Motonari Uesugi
Journal:  Nucleic Acids Res       Date:  2022-08-12       Impact factor: 19.160

6.  CNBP Binds and Unfolds In Vitro G-Quadruplexes Formed in the SARS-CoV-2 Positive and Negative Genome Strands.

Authors:  Georgina Bezzi; Ernesto J Piga; Andrés Binolfi; Pablo Armas
Journal:  Int J Mol Sci       Date:  2021-03-05       Impact factor: 5.923

7.  Mechanical insights into ribosomal progression overcoming RNA G-quadruplex from periodical translation suppression in cells.

Authors:  Tamaki Endoh; Naoki Sugimoto
Journal:  Sci Rep       Date:  2016-03-07       Impact factor: 4.379

8.  Reconstitution of a functional 7SK snRNP.

Authors:  John E Brogie; David H Price
Journal:  Nucleic Acids Res       Date:  2017-06-20       Impact factor: 16.971

9.  RNA G-quadruplex structures exist and function in vivo in plants.

Authors:  Xiaofei Yang; Jitender Cheema; Yueying Zhang; Hongjing Deng; Susan Duncan; Mubarak Ishaq Umar; Jieyu Zhao; Qi Liu; Xiaofeng Cao; Chun Kit Kwok; Yiliang Ding
Journal:  Genome Biol       Date:  2020-09-01       Impact factor: 13.583
  9 in total

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