| Literature DB >> 20206170 |
Ying Wang1, Masahiro Nishi, Asako Doi, Takeshi Shono, Yasushi Furukawa, Takeshi Shimada, Hiroto Furuta, Hideyuki Sasaki, Kishio Nanjo.
Abstract
Ghrelin inhibits insulin secretion partly via induction of IA-2beta. However, the orexigenic effect of ghrelin is mediated by the AMP-activated protein kinase (AMPK)-uncoupling protein 2 (UCP2) pathway. Here, we demonstrate that ghrelin's inhibitory effect on insulin secretion also occurs through the AMPK-UCP2 pathway. Ghrelin increased AMPK phosphorylation and UCP2 mRNA expression in MIN6 insulinoma cells. Overexpression or downregulation of UCP2 attenuated or enhanced insulin secretion, respectively. Furthermore, AMPK activator had a similar effect to ghrelin on UCP2 and insulin secretion in MIN6 cells. In conclusion, ghrelin's inhibitory effect on insulin secretion is partly mediated by the AMPK-UCP2 pathway, which is independent of the IA-2beta pathway. Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.Entities:
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Year: 2010 PMID： 20206170 DOI： 10.1016/j.febslet.2010.02.069
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124