Xu-fei Tan1, Feng Chen, Shan-shan Wu, Yu Shi, Dong-cheng Liu, Zhi Chen. 1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
Abstract
OBJECTIVE: To find new protein biomarkers for the detection and evaluation of liver injury and to analyze the relationship between such proteins and disease progression in concanavalin A (Con A)-induced hepatitis. METHODS: Twenty-five mice were randomly divided into five groups: an untreated group, a control group injected with phosphate buffered saline (PBS), and groups with Con A-induced hepatitis evaluated at 1, 3 and 6 h. Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify differences in protein expression among groups. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the results. RESULTS: In mice with Con A-induced hepatitis, expression levels of four proteins were increased: RIKEN, fructose bisphosphatase 1 (fbp1), ketohexokinase (khk), and Chain A of class pi glutathione S-transferase. Changes in fbp1 and khk were confirmed by qRT-PCR. CONCLUSION: Levels of two proteins, fbp1 and khk, are clearly up-regulated in mice with Con A-induced hepatitis.
OBJECTIVE: To find new protein biomarkers for the detection and evaluation of liver injury and to analyze the relationship between such proteins and disease progression in concanavalin A (Con A)-induced hepatitis. METHODS: Twenty-five mice were randomly divided into five groups: an untreated group, a control group injected with phosphate buffered saline (PBS), and groups with Con A-induced hepatitis evaluated at 1, 3 and 6 h. Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify differences in protein expression among groups. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the results. RESULTS: In mice with Con A-induced hepatitis, expression levels of four proteins were increased: RIKEN, fructose bisphosphatase 1 (fbp1), ketohexokinase (khk), and Chain A of class pi glutathione S-transferase. Changes in fbp1 and khk were confirmed by qRT-PCR. CONCLUSION: Levels of two proteins, fbp1 and khk, are clearly up-regulated in mice with Con A-induced hepatitis.
Authors: Robert N McBurney; Wade M Hines; Linda S Von Tungeln; Laura K Schnackenberg; Richard D Beger; Carrie L Moland; Tao Han; James C Fuscoe; Ching-Wei Chang; James J Chen; Zhenqiang Su; Xiao-Hui Fan; Weida Tong; Shelagh A Booth; Raji Balasubramanian; Paul L Courchesne; Jennifer M Campbell; Armin Graber; Yu Guo; Peter J Juhasz; Tricin Y Li; Moira D Lynch; Nicole M Morel; Thomas N Plasterer; Edward J Takach; Chenhui Zeng; Frederick A Beland Journal: Toxicol Pathol Date: 2009-01-26 Impact factor: 1.902
Authors: Qing-Yu He; George K K Lau; Yuan Zhou; Siu-Tsan Yuen; Marie C Lin; Hsiang-Fu Kung; Jen-Fu Chiu Journal: Proteomics Date: 2003-05 Impact factor: 3.984