Literature DB >> 20204523

Rapid separation of human globin chains in normal and thalassemia patients by RP-HPLC.

Hooshang Nemati1, Gholamreza Bahrami, Zohreh Rahimi.   

Abstract

The present study describes a rapid and sensitive high-performance liquid chromatography (HPLC) method for the detection of human globin chains in blood. The method involves direct injection of globin chains which prepared by a standard method onto a micro bondapack C18 reversed-phase column (7.8 mm I.D.) with UV detection at 280 nm. The detection limit of hemoglobin (Hb) was 0.1 μg, which is equivalent to about 1 ml of fresh whole blood. We report here the rapid procedure for globin chain analysis. The present method will be useful for the determination of globin chain analysis in clinical laboratories, as well as in thalassemia and sickle cell disease patients.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20204523     DOI： 10.1007/s11033-010-9994-4

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  16 in total

Review 1.  Abnormal hemoglobins: laboratory methods.

Authors:  H Wajcman; C Préhu; J Bardakdjian-Michau; D Promé; J Riou; C Godart; M Mathis; D Hurtrel; F Galactéros
Journal:  Hemoglobin       Date:  2001-05       Impact factor: 0.849

2.  Analysis of hemoglobins and globin chains by high-performance liquid chromatography.

Authors:  Henri Wajcman
Journal:  Methods Mol Med       Date:  2003

3.  Beta-globin gene cluster haplotypes in sickle cell patients from southwest Iran.

Authors:  Z Rahimi; M Karimi; M Haghshenass; A Merat
Journal:  Am J Hematol       Date:  2003-11       Impact factor: 10.047

4.  THE AMINO ACID SEQUENCE OF THE GAMMA CHAIN OF HUMAN FETAL HEMOGLOBIN.

Authors:  W A SCHROEDER; J R SHELTON; J B SHELTON; J CORMICK; R T JONES
Journal:  Biochemistry       Date:  1963 Sep-Oct       Impact factor: 3.162

5.  Implications of the genetic epidemiology of globin haplotypes linked to the sickle cell gene in southern Iran.

Authors:  Zohreh Rahimi; Ahmad Merat; Nathalie Gerard; Rajagopal Krishnamoorthy; Ronald L Nagel
Journal:  Hum Biol       Date:  2006-12       Impact factor: 0.553

6.  Separation of haemoglobin HbE and HbA by the fully automated, high-pressure liquid chromatography Tosoh HLC-723 G7 analyzer.

Authors:  G Lippi; M R Carta; G L Salvagno; F Bellorio; M Montagnana; G Soffiati; G C Guidi
Journal:  Int J Lab Hematol       Date:  2008-10       Impact factor: 2.877

7.  Sensitive detection of human globin chains by microbore high-performance liquid chromatography and its forensic application.

Authors:  H Inoue; Y Maeno; M Iwasa; H Koyama; Y Sato; R Matoba
Journal:  J Chromatogr B Biomed Sci Appl       Date:  1997-01-24

8.  Differentiation of electrophoretically similar hemoglobins--such as S, D, G, and P; or A2, C, E, and O--by electrophoresis of the globin chains.

Authors:  R G Schneider
Journal:  Clin Chem       Date:  1974-09       Impact factor: 8.327

9.  Prenatal diagnosis of beta-thalassemia. Experience with 24 cases.

Authors:  M Furbetta; A Angius; A Ximenes; R Fais; A Cao; C Valenti; P Fioretti; F Caminiti; G Angioni; A Nasi
Journal:  Isr J Med Sci       Date:  1978-11

10.  The Xmn1 polymorphic site 5' to the (G)gamma gene and its correlation to the (G)gamma:(A)gamma ratio, age at first blood transfusion and clinical features in beta-thalassemia patients from Western Iran.

Authors:  Hooshang Nemati; Zohreh Rahimi; Gholamreza Bahrami
Journal:  Mol Biol Rep       Date:  2009-05-15       Impact factor: 2.316
View more
  4 in total

1.  Gene replacement of α-globin with β-globin restores hemoglobin balance in β-thalassemia-derived hematopoietic stem and progenitor cells.

Authors:  M Kyle Cromer; Joab Camarena; Renata M Martin; Benjamin J Lesch; Christopher A Vakulskas; Nicole M Bode; Gavin Kurgan; Michael A Collingwood; Garrett R Rettig; Mark A Behlke; Viktor T Lemgart; Yankai Zhang; Ankush Goyal; Feifei Zhao; Ezequiel Ponce; Waracharee Srifa; Rasmus O Bak; Naoya Uchida; Ravindra Majeti; Vivien A Sheehan; John F Tisdale; Daniel P Dever; Matthew H Porteus
Journal:  Nat Med       Date:  2021-03-18       Impact factor: 53.440

2.  Hemoglobin D-Punjab: origin, distribution and laboratory diagnosis.

Authors:  Lidiane de Souza Torres; Jéssika Viviani Okumura; Danilo Grünig Humberto da Silva; Claudia Regina Bonini-Domingos
Journal:  Rev Bras Hematol Hemoter       Date:  2015-02-23

3.  Rapid and Sensitive Assessment of Globin Chains for Gene and Cell Therapy of Hemoglobinopathies.

Authors:  Constantinos C Loucari; Petros Patsali; Thamar B van Dijk; Coralea Stephanou; Panayiota Papasavva; Maria Zanti; Ryo Kurita; Yukio Nakamura; Soteroulla Christou; Maria Sitarou; Sjaak Philipsen; Carsten W Lederer; Marina Kleanthous
Journal:  Hum Gene Ther Methods       Date:  2018-02       Impact factor: 2.396

4.  Highly efficient editing of the β-globin gene in patient-derived hematopoietic stem and progenitor cells to treat sickle cell disease.

Authors:  So Hyun Park; Ciaran M Lee; Daniel P Dever; Timothy H Davis; Joab Camarena; Waracharee Srifa; Yankai Zhang; Alireza Paikari; Alicia K Chang; Matthew H Porteus; Vivien A Sheehan; Gang Bao
Journal:  Nucleic Acids Res       Date:  2019-09-05       Impact factor: 16.971
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.