OBJECTIVE: Stressful exercise reduces the plasma pro-inflammatory cytokine concentration in response to lipopolysaccharide (LPS). The aim of this study was to clarify the mechanism of exhaustive exercise-induced suppression of the plasma tumor necrosis factor (TNF)-alpha concentration in response to LPS. METHODS: Male C3H/HeN mice (n = 66) were randomized to treadmill running to exhaustion (Ex) or a sedentary (Non-Ex) condition. Monocytes and splenic macrophages were collected from some animals, and other animals were injected with LPS (1 mg/kg) immediately after the exercise. The liver, lung and spleen tissues in the mice were removed 30 min after the LPS injection for determination of TNF-alpha mRNA expression. Blood and tissue samples were collected for determination of TNF-alpha and TNF receptors (TNFR) 1 h after the LPS injection. RESULTS: Although there was a significant suppression in LPS-induced plasma TNF-alpha in the Ex mice when compared to the Non-Ex mice (p < 0.01), soluble TNFR in plasma was not affected by the exercise. There was no change in cell-surface expression of Toll-like receptor 4 (TLR4) and in LPS-induced TNF-alpha mRNA expression and TNFR content in tissues between the Ex and Non-Ex groups. Interestingly, TNF-alpha contents in the liver, lung and spleen of the Ex mice were significantly lower than those of the Non-Ex group (p < 0.01, p < 0.01 and p < 0.05, respectively). CONCLUSION: These data suggest that exhaustive exercise-induced suppression of the plasma TNF-alpha concentration despite LPS stimulation might depend on translation of TNF-alpha in tissues.
OBJECTIVE: Stressful exercise reduces the plasma pro-inflammatory cytokine concentration in response to lipopolysaccharide (LPS). The aim of this study was to clarify the mechanism of exhaustive exercise-induced suppression of the plasma tumor necrosis factor (TNF)-alpha concentration in response to LPS. METHODS: Male C3H/HeN mice (n = 66) were randomized to treadmill running to exhaustion (Ex) or a sedentary (Non-Ex) condition. Monocytes and splenic macrophages were collected from some animals, and other animals were injected with LPS (1 mg/kg) immediately after the exercise. The liver, lung and spleen tissues in the mice were removed 30 min after the LPS injection for determination of TNF-alpha mRNA expression. Blood and tissue samples were collected for determination of TNF-alpha and TNF receptors (TNFR) 1 h after the LPS injection. RESULTS: Although there was a significant suppression in LPS-induced plasma TNF-alpha in the Ex mice when compared to the Non-Ex mice (p < 0.01), soluble TNFR in plasma was not affected by the exercise. There was no change in cell-surface expression of Toll-like receptor 4 (TLR4) and in LPS-induced TNF-alpha mRNA expression and TNFR content in tissues between the Ex and Non-Ex groups. Interestingly, TNF-alpha contents in the liver, lung and spleen of the Ex mice were significantly lower than those of the Non-Ex group (p < 0.01, p < 0.01 and p < 0.05, respectively). CONCLUSION: These data suggest that exhaustive exercise-induced suppression of the plasma TNF-alpha concentration despite LPS stimulation might depend on translation of TNF-alpha in tissues.
Authors: Stephen A Martin; Brandt D Pence; Ryan M Greene; Stephanie J Johnson; Robert Dantzer; Keith W Kelley; Jeffrey A Woods Journal: Brain Behav Immun Date: 2012-12-28 Impact factor: 7.217
Authors: Yvonne Huber; Nadine Gehrke; Jana Biedenbach; Susanne Helmig; Perikles Simon; Beate K Straub; Ina Bergheim; Tobias Huber; Detlef Schuppan; Peter R Galle; Marcus A Wörns; Marcus Schuchmann; Jörn M Schattenberg Journal: Cell Death Dis Date: 2017-06-22 Impact factor: 8.469
Authors: Vanessa Frodermann; David Rohde; Gabriel Courties; Nicolas Severe; Maximilian J Schloss; Hajera Amatullah; Cameron S McAlpine; Sebastian Cremer; Friedrich F Hoyer; Fei Ji; Ian D van Koeverden; Fanny Herisson; Lisa Honold; Gustavo Santos Masson; Shuang Zhang; Jana Grune; Yoshiko Iwamoto; Stephen P Schmidt; Gregory R Wojtkiewicz; I-Hsiu Lee; Karin Gustafsson; Gerard Pasterkamp; Saskia C A de Jager; Ruslan I Sadreyev; Jean MacFadyen; Peter Libby; Paul Ridker; David T Scadden; Kamila Naxerova; Kate L Jeffrey; Filip K Swirski; Matthias Nahrendorf Journal: Nat Med Date: 2019-11-07 Impact factor: 53.440