OBJECTIVE: Our aim was to validate the use of tissue microarrays (TMA) in oral squamous cell carcinomas (OSCC) to analyse epidermal growth factor receptor (EGFR) and Ki-67 expression. We also analysed the relationship that the expression of these markers may have with clinical, pathological and survival variables. PATIENTS AND METHODS: The study sample comprised 39 unselected patients diagnosed and treated for OSCC. We analysed Ki-67 and EGFR expression by immunohistochemistry on formalin-fixed, paraffin-embedded surgical specimens. Whole sections (WS) were compared with double 1.5 mm core-tissue microarrays. RESULTS: High EGFR expression was observed both on TMA (in 98% of the cases) and WS (in 100% of the cases) with substantial agreement kappa value (0.720). EGFR expression was not significantly associated with clinical, pathological and survival variables on TMA and WS. Ki-67 analysis showed a Spearman correlation of 0.741 with a Ki-67 mean labelling index of 45% in TMA and 56.8% in WS. We found a significant relationship between gender and Ki-67 labelling index on WS (P = 0.022) and TMA (P = 0.002). Clinical stage was the only parameter in multivariate analysis that had a significant predictive value. CONCLUSION: We demonstrate that dual 1.5 mm core TMA is a valid, rapid, economical and tissue-saving way to study OSCC biopsies and that it presents strong correlation with the WS. EGFR overexpression in OSCC suggests that these tumours may be a candidate for therapy investigation directed to EGFR.
OBJECTIVE: Our aim was to validate the use of tissue microarrays (TMA) in oral squamous cell carcinomas (OSCC) to analyse epidermal growth factor receptor (EGFR) and Ki-67 expression. We also analysed the relationship that the expression of these markers may have with clinical, pathological and survival variables. PATIENTS AND METHODS: The study sample comprised 39 unselected patients diagnosed and treated for OSCC. We analysed Ki-67 and EGFR expression by immunohistochemistry on formalin-fixed, paraffin-embedded surgical specimens. Whole sections (WS) were compared with double 1.5 mm core-tissue microarrays. RESULTS: High EGFR expression was observed both on TMA (in 98% of the cases) and WS (in 100% of the cases) with substantial agreement kappa value (0.720). EGFR expression was not significantly associated with clinical, pathological and survival variables on TMA and WS. Ki-67 analysis showed a Spearman correlation of 0.741 with a Ki-67 mean labelling index of 45% in TMA and 56.8% in WS. We found a significant relationship between gender and Ki-67 labelling index on WS (P = 0.022) and TMA (P = 0.002). Clinical stage was the only parameter in multivariate analysis that had a significant predictive value. CONCLUSION: We demonstrate that dual 1.5 mm core TMA is a valid, rapid, economical and tissue-saving way to study OSCC biopsies and that it presents strong correlation with the WS. EGFR overexpression in OSCC suggests that these tumours may be a candidate for therapy investigation directed to EGFR.
Authors: Nikolina Dioufa; Elena Farmaki; Andrew V Schally; Hippokratis Kiaris; Dimitris Vlahodimitropoulos; Athanasios G Papavassiliou; Christos Kittas; Norman L Block; Ioulia Chatzistamou Journal: Horm Cancer Date: 2012-03-23 Impact factor: 3.869
Authors: John B McIntyre; Pinaki Bose; Alexander C Klimowicz; Nigel T Brockton; Stephanie Petrillo; Wayne Matthews; Jay Easaw; Anthony Magliocco; Joseph C Dort Journal: PLoS One Date: 2012-02-16 Impact factor: 3.240
Authors: Wattawan Wongpattaraworakul; Katherine N Gibson-Corley; Allen Choi; Marisa R Buchakjian; Emily A Lanzel; Anand Rajan Kd; Andrean L Simons Journal: Front Oncol Date: 2022-07-25 Impact factor: 5.738
Authors: Luís Silva Monteiro; Maria Leonor Delgado; Sara Ricardo; Fernanda Garcez; Barbas do Amaral; José Júlio Pacheco; Carlos Lopes; Hassan Bousbaa Journal: Biomed Res Int Date: 2014-05-21 Impact factor: 3.411