Literature DB >> 20201527

Flow induced dispersion analysis quantifies noncovalent interactions in nanoliter samples.

Henrik Jensen1, Jesper Østergaard.   

Abstract

Flow Induced Dispersion Analysis (FIDA) is presented as a new approach to characterize noncovalent interactions. The new method only requires nanoliter samples, is easy to implement, and also provides diffusivities of the free analyte and the formed complex. The technique is based on signal analysis of temporal variances obtained for pressure driven flows in micrometer sized fused silica capillaries.

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Year:  2010        PMID： 20201527     DOI： 10.1021/ja100484d

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  9 in total

1.  Immobilization-Free Binding and Affinity Characterization of Higher Order Bispecific Antibody Complexes Using Size-Based Microfluidics.

Authors:  Andreas V Madsen; Oscar Mejias-Gomez; Lasse E Pedersen; Kerstin Skovgaard; Peter Kristensen; Steffen Goletz
Journal:  Anal Chem       Date:  2022-09-27       Impact factor: 8.008

2.  Quantification of Structural Integrity and Stability Using Nanograms of Protein by Flow-Induced Dispersion Analysis.

Authors:  Morten E Pedersen; Jesper Østergaard; Henrik Jensen
Journal:  Molecules       Date:  2022-04-13       Impact factor: 4.927

3.  Biochemical Investigation of the Interaction of pICln, RioK1 and COPR5 with the PRMT5-MEP50 Complex.

Authors:  Adrian Krzyzanowski; Raphael Gasper; Hélène Adihou; Peter 't Hart; Herbert Waldmann
Journal:  Chembiochem       Date:  2021-03-31       Impact factor: 3.164

4.  Measuring Self-Association of Antibody Lead Candidates with Dynamic Light Scattering.

Authors:  Fabian Dingfelder; Anette Henriksen; Per-Olof Wahlund; Paolo Arosio; Nikolai Lorenzen
Journal:  Methods Mol Biol       Date:  2022

5.  In-Solution IgG Titer Determination in Fermentation Broth Using Affibodies and Flow-Induced Dispersion Analysis.

Authors:  Morten E Pedersen; Jesper Østergaard; Henrik Jensen
Journal:  ACS Omega       Date:  2020-04-28

6.  Size-based characterization of adalimumab and TNF-α interactions using flow induced dispersion analysis: assessment of avidity-stabilized multiple bound species.

Authors:  Morten E Pedersen; Ragna M S Haegebaert; Jesper Østergaard; Henrik Jensen
Journal:  Sci Rep       Date:  2021-02-26       Impact factor: 4.379

7.  Capillary flow experiments for thermodynamic and kinetic characterization of protein liquid-liquid phase separation.

Authors:  Emil G P Stender; Soumik Ray; Rasmus K Norrild; Jacob Aunstrup Larsen; Daniel Petersen; Azad Farzadfard; Céline Galvagnion; Henrik Jensen; Alexander K Buell
Journal:  Nat Commun       Date:  2021-12-15       Impact factor: 14.919

8.  Assessment of immunogenicity and drug activity in patient sera by flow-induced dispersion analysis.

Authors:  Morten E Pedersen; Jesper Østergaard; Bente Glintborg; Merete L Hetland; Henrik Jensen
Journal:  Sci Rep       Date:  2022-03-18       Impact factor: 4.379

9.  A serum-stable RNA aptamer specific for SARS-CoV-2 neutralizes viral entry.

Authors:  Julián Valero; Laia Civit; Daniel M Dupont; Denis Selnihhin; Line S Reinert; Manja Idorn; Brett A Israels; Aleksandra M Bednarz; Claus Bus; Benedikt Asbach; David Peterhoff; Finn S Pedersen; Victoria Birkedal; Ralf Wagner; Søren R Paludan; Jørgen Kjems
Journal:  Proc Natl Acad Sci U S A       Date:  2021-12-14       Impact factor: 11.205
  9 in total

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