Sarah Wallbutton1, Jason Kasraie. 1. Shropshire & Mid-Wales Fertility Centre, Shrewsbury and Telford Hospitals NHS Trust, Mytton Oak Road, Shrewsbury, Shropshire, SY3 8XQ, UK.
Abstract
PURPOSE: To describe a case of homogeneous macro vacuolar formation in oocytes from a patient undergoing IVF/ICSI over 3 cycles and implications thereof. METHODS: Case report and analysis of cycle outcomes including spindle visualization to determine severity of internal disruption caused. RESULTS: Characteristic macro vacuoles (>25 microm diameter) were identified in the vast majority of oocytes. Spindle visualization identified that macro vacuoles were not associated with spindle displacement. IVF resulted in failed fertilization. Low numbers of fertilized oocytes were obtained through ICSI. The vast majority of embryos exhibited maturation arrest on day 2. CONCLUSIONS: It is likely that persistent Oocyte Macro Vacuolization is the cause of infertility in this patient. It probably has a biological basis and possibly a genetic cause, resulting in either uncontrollable endocytosis or poor exocytosis and consequent vacuolar formation.
PURPOSE: To describe a case of homogeneous macro vacuolar formation in oocytes from a patient undergoing IVF/ICSI over 3 cycles and implications thereof. METHODS: Case report and analysis of cycle outcomes including spindle visualization to determine severity of internal disruption caused. RESULTS: Characteristic macro vacuoles (>25 microm diameter) were identified in the vast majority of oocytes. Spindle visualization identified that macro vacuoles were not associated with spindle displacement. IVF resulted in failed fertilization. Low numbers of fertilized oocytes were obtained through ICSI. The vast majority of embryos exhibited maturation arrest on day 2. CONCLUSIONS: It is likely that persistent Oocyte Macro Vacuolization is the cause of infertility in this patient. It probably has a biological basis and possibly a genetic cause, resulting in either uncontrollable endocytosis or poor exocytosis and consequent vacuolar formation.
Authors: Emanuela Lazzaroni-Tealdi; David H Barad; David F Albertini; Yao Yu; Vitaly A Kushnir; Helena Russell; Yan-Guang Wu; Norbert Gleicher Journal: PLoS One Date: 2015-12-02 Impact factor: 3.240