PROBLEM: To investigate the contribution of genomic variations in the indoleamine 2,3-dioxygenase (IDO) gene to the onset of pre-eclampsia. METHOD OF STUDY: We examined sequence variations in the IDO1 gene using placental genomic DNA from 35 pre-eclamptic patients and 32 normotensive pregnant women. RESULTS: A case-control study revealed that none of the common variants influences the risk of disease. Sequencing of each IDO1 exon in diseased subjects revealed rare variants. This variation, c.-147_150delGAAA, was located within the 5'-untranslated region of the IDO1 gene, and its homozygote was identified only in pre-eclamptic subjects. However, despite the low levels of IDO expression and enzyme activity in the c.-147_150delGAAA homozygote, reporter assays indicated that this variation does not affect gene expression. CONCLUSION: Our findings indicate that genetic alteration of fetal IDO gene does not appear to be a primary cause of pre-eclampsia.
PROBLEM: To investigate the contribution of genomic variations in the indoleamine 2,3-dioxygenase (IDO) gene to the onset of pre-eclampsia. METHOD OF STUDY: We examined sequence variations in the IDO1 gene using placental genomic DNA from 35 pre-eclamptic patients and 32 normotensive pregnant women. RESULTS: A case-control study revealed that none of the common variants influences the risk of disease. Sequencing of each IDO1 exon in diseased subjects revealed rare variants. This variation, c.-147_150delGAAA, was located within the 5'-untranslated region of the IDO1 gene, and its homozygote was identified only in pre-eclamptic subjects. However, despite the low levels of IDO expression and enzyme activity in the c.-147_150delGAAA homozygote, reporter assays indicated that this variation does not affect gene expression. CONCLUSION: Our findings indicate that genetic alteration of fetal IDO gene does not appear to be a primary cause of pre-eclampsia.
Authors: Sook-Eun Park; Marcus Lawson; Robert Dantzer; Keith W Kelley; Robert H McCusker Journal: J Neuroinflammation Date: 2011-12-21 Impact factor: 8.322
Authors: Alexander Lee; Navya Kanuri; Yuanhao Zhang; Gregory S Sayuk; Ellen Li; Matthew A Ciorba Journal: PLoS One Date: 2014-12-26 Impact factor: 3.240