BACKGROUND: The goal of this study was to investigate the roles of PIM-1 in prostate cancer (CaP) cell proliferation and apoptosis, and to assess the potential of PIM-1 as a target for CaP therapy. METHODS: Using RNAi technology, we knocked down the expression of PIM-1 in PC-3 cell. After siRNA transfection, cell morphology, cell proliferation, cell cycle, and apoptosis rate were analyzed. PIM-1 siRNA with Lipofectamine were injected into xenograft models to evaluate its therapeutic effect. RESULTS: PIM-1 siRNA significantly inhibited PIM-1 expression. In vitro, silencing of the PIM-1 gene resulted in irregular cell morphology, decreased cell proliferation, inhibition of cell-cycle progression, and induction of apoptosis. Compared with control groups, intratumoral injection of PIM-1 siRNA with Lipofectamine in nude mice dramatically suppressed PC-3 tumor progression. CONCLUSIONS: PIM-1 could play important roles in the progression of CaP and may be an interesting target for CaP therapy. (c) 2010 Wiley-Liss, Inc.
BACKGROUND: The goal of this study was to investigate the roles of PIM-1 in prostate cancer (CaP) cell proliferation and apoptosis, and to assess the potential of PIM-1 as a target for CaP therapy. METHODS: Using RNAi technology, we knocked down the expression of PIM-1 in PC-3 cell. After siRNA transfection, cell morphology, cell proliferation, cell cycle, and apoptosis rate were analyzed. PIM-1 siRNA with Lipofectamine were injected into xenograft models to evaluate its therapeutic effect. RESULTS:PIM-1 siRNA significantly inhibited PIM-1 expression. In vitro, silencing of the PIM-1 gene resulted in irregular cell morphology, decreased cell proliferation, inhibition of cell-cycle progression, and induction of apoptosis. Compared with control groups, intratumoral injection of PIM-1 siRNA with Lipofectamine in nude mice dramatically suppressed PC-3 tumor progression. CONCLUSIONS:PIM-1 could play important roles in the progression of CaP and may be an interesting target for CaP therapy. (c) 2010 Wiley-Liss, Inc.
Authors: Yong Xu; Benjamin G Brenning; Steven G Kultgen; Jason M Foulks; Adrianne Clifford; Shuping Lai; Ashley Chan; Shannon Merx; Michael V McCullar; Steven B Kanner; Koc-Kan Ho Journal: ACS Med Chem Lett Date: 2014-10-22 Impact factor: 4.345
Authors: Maja Narlik-Grassow; Carmen Blanco-Aparicio; Yolanda Cecilia; Marco Perez; Sandra Muñoz-Galvan; Marta Cañamero; Oliver Renner; Amancio Carnero Journal: PLoS One Date: 2013-04-02 Impact factor: 3.240