A novel flow-injection chemiluminescence determination of uric acid based on diperiodatoargentate(III) oxidation.

A novel and high selectivity flow-injection chemiluminescence (FI-CL) system with diperiodatoargentate(III) (DPA) is developed for the determination of uric acid for the first time. It is based on the reaction of diperiodatoargentate(III) (DPA) with uric acid in alkaline medium to emit CL. With the peak height as a quantitative parameter applying optimum working conditions, the relative CL intensity was linear with the uric acid concentration in the range of 4.0 x 10(-7)-2.0 x 10(-4) mol L(-1) with a detection limit of 1.2 x 10(-7) mol L(-1) (3 sigma). The relative standard deviation (RSD) was 2.1% for 5.0 x 10(-5) mol L(-1) uric acid (n=7). The proposed method held higher selectivity than other CL methods and was applied to determination of uric acid in human serum. The possible CL reaction mechanism was also discussed briefly. (c) 2009 Elsevier B.V. All rights reserved.