OBJECTIVES: Recent research has focused on application of growth factors such as bone morphogenetic proteins (BMPs) as alternatives to autogenous bone grafting. Two bone graft substitute bioimplants containing recombinant human BMPs (rhBMPs), Infuse (rhBMP-2) and OP-1 (rhBMP-7), are approved for human application but have never been compared side by side. The aim of this study was to provide a direct comparison of the osteoinductive activity of the 2 commercially available and approved rhBMP-containing bioimplants in their clinically available forms. STUDY DESIGN: The activity of rhBMP-2 and -7 in solution were compared in vitro using the C2C12 cell-based assay. The activity of Infuse and OP-1 bioimplants containing 52.5 microg of rhBMP-2 or rhBMP-7, respectively, were compared in vivo using a mouse muscle pouch assay and analyzed by microscopic CT (microCT) and histology. RESULTS: The in vitro results showed that rhBMP-2 stimulated greater alkaline phosphatase production than rhBMP-7 over various time points and concentrations. The in vivo results showed that OP-1 induced greater bone volume than Infuse. Both implants induced bone of equivalent quality based on microCT and histologic evaluation. CONCLUSION: In their clinically available forms, the rhBMP-7-containing OP-1 induced greater bone volume than the rhBMP-2-containing Infuse in the mouse muscle pouch model. Copyright 2010 Mosby, Inc. All rights reserved.
OBJECTIVES: Recent research has focused on application of growth factors such as bone morphogenetic proteins (BMPs) as alternatives to autogenous bone grafting. Two bone graft substitute bioimplants containing recombinant human BMPs (rhBMPs), Infuse (rhBMP-2) and OP-1 (rhBMP-7), are approved for human application but have never been compared side by side. The aim of this study was to provide a direct comparison of the osteoinductive activity of the 2 commercially available and approved rhBMP-containing bioimplants in their clinically available forms. STUDY DESIGN: The activity of rhBMP-2 and -7 in solution were compared in vitro using the C2C12 cell-based assay. The activity of Infuse and OP-1 bioimplants containing 52.5 microg of rhBMP-2 or rhBMP-7, respectively, were compared in vivo using a mouse muscle pouch assay and analyzed by microscopic CT (microCT) and histology. RESULTS: The in vitro results showed that rhBMP-2 stimulated greater alkaline phosphatase production than rhBMP-7 over various time points and concentrations. The in vivo results showed that OP-1 induced greater bone volume than Infuse. Both implants induced bone of equivalent quality based on microCT and histologic evaluation. CONCLUSION: In their clinically available forms, the rhBMP-7-containing OP-1 induced greater bone volume than the rhBMP-2-containing Infuse in the mouse muscle pouch model. Copyright 2010 Mosby, Inc. All rights reserved.
Authors: Suzanne N Lissenberg-Thunnissen; David J J de Gorter; Cornelis F M Sier; Inger B Schipper Journal: Int Orthop Date: 2011-06-23 Impact factor: 3.075
Authors: Jan Wolff; George K Sándor; Aimo Miettinen; Veikko J Tuovinen; Bettina Mannerström; Mimmi Patrikoski; Susanna Miettinen Journal: Ann Maxillofac Surg Date: 2013-07
Authors: Adelina A Hernandez-Hurtado; Gissela Borrego-Soto; Ivan A Marino-Martinez; Jorge Lara-Arias; Viktor J Romero-Diaz; Adalberto Abrego-Guerra; Jose F Vilchez-Cavazos; Guillermo Elizondo-Riojas; Herminia G Martinez-Rodriguez; Marcela A Espinoza-Juarez; Gloria C Lopez-Romero; Alejandro Robles-Zamora; Oscar F Mendoza Lemus; Rocio Ortiz-Lopez; Augusto Rojas-Martinez Journal: Stem Cells Int Date: 2016-10-13 Impact factor: 5.443