Identification of the ATP-binding heat-inducible protein of MR = 37,000 as glyceraldehyde-3-phosphate dehydrogenase.

We previously found a novel ATP-binding heat-inducible protein of Mr = 37,000 in BALB/c 3T3 cells. Here, we found that the peptide mapping of this 37-kDa protein was similar to that of rabbit glyceraldehyde-3-phosphate dehydrogenase. Therefore, we biochemically compared the 37-kDa protein with a product translated from mRNA which was hybrid-selected using a cDNA for encoding chick glyceraldehyde-3-phosphate dehydrogenase and found that these two proteins were very similar. Northern blotting analysis using its cDNA as a probe revealed that glyceraldehyde-3-phosphate dehydrogenase was a heat-inducible protein in BALB/c 3T3 cells and that it was induced by stresses including treatment with alpha, alpha'-dipyridyl.