| Literature DB >> 20181906 |
Krishna K Gopaul1, Jessica Sells, Betsy J Bricker, Oswald R Crasta, Adrian M Whatmore.
Abstract
The reliable differentiation of live Brucella vaccine strains from field isolates is an important element in brucellosis control programs. We describe the design, validation, and implementation of a novel single nucleotide polymorphism (SNP)-based typing platform that offers a rapid, reliable, and robust tool to achieve this with improved diagnostic accuracy compared to existing molecular tests. Furthermore, the assays described are designed such that they supplement, and can be run as an intrinsic part of, a previously described assay identifying Brucella isolates to the species level (K. K. Gopaul, C. J. Smith, M. S. Koylass, and A. M. Whatmore, BMC Microbiol. 8:86), giving a comprehensive molecular typing platform.Entities:
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Year: 2010 PMID: 20181906 PMCID: PMC2849582 DOI: 10.1128/JCM.02193-09
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948