Literature DB >> 20177994

Derivation of Man-1 and Man-2 research grade human embryonic stem cell lines.

Maria V Camarasa1, Robbie W Kerr, Sharon F Sneddon, Nicola Bates, Lisa Shaw, Rachel A Oldershaw, Fiona Small, Melissa A Baxter, Tristan R Mckay, Daniel R Brison, Susan J Kimber.   

Abstract

We report here the derivation of two new human embryonic stem cell lines, Man-1 and Man-2, and their full characterization as novel pluripotent stem cell lines. Man-1 was derived from an embryo surplus to requirement from routine IVF, while Man-2 was obtained from an oocyte classified as failed to fertilise and subsequently chemically activated. We report the characterisation of pluripotency and the differentiation potential of these lines. Work is in progress to establish novel methods of stem cell derivation and culture, which will avoid the use of xenobiotics and be relevant to clinical production of human embryonic stem cell lines. Both newly derived human embryonic stem cell lines will be available for the research community from the UK Stem Cell Bank (http://www.ukstemcellbank.org.uk).

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Year:  2010        PMID： 20177994     DOI： 10.1007/s11626-010-9291-5

Source DB:  PubMed          Journal:  In Vitro Cell Dev Biol Anim        ISSN： 1071-2690            Impact factor:   2.416


  31 in total

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4.  Immortalized feeders for the scale-up of human embryonic stem cells in feeder and feeder-free conditions.

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Journal:  J Biotechnol       Date:  2005-10-17       Impact factor: 3.307

5.  Human feeder layers for human embryonic stem cells.

Authors:  M Amit; V Margulets; H Segev; K Shariki; I Laevsky; R Coleman; J Itskovitz-Eldor
Journal:  Biol Reprod       Date:  2003-01-22       Impact factor: 4.285

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Authors:  Mavi Camarasa; Daniel Brison; Susan J Kimber; Alan H Handyside
Journal:  Cloning Stem Cells       Date:  2009-09

7.  Embryonic stem cell lines derived from human blastocysts.

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8.  Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells.

Authors:  Mark Richards; Shawna Tan; Chui-Yee Fong; Arjit Biswas; Woon-Khiong Chan; Ariff Bongso
Journal:  Stem Cells       Date:  2003       Impact factor: 6.277

9.  Clinically failed eggs as a source of normal human embryo stem cells.

Authors:  Paul A De Sousa; John Gardner; Sharon Sneddon; Steve Pells; Britt Jorgensen Tye; Pawlina Dand; Daniel M Collins; Karen Stewart; Lisa Shaw; Stefan Przyborski; Michael Cooke; K John McLaughlin; Susan J Kimber; Brian A Lieberman; Ian Wilmut; Daniel R Brison
Journal:  Stem Cell Res       Date:  2009-02-06       Impact factor: 2.020

10.  Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture.

Authors:  Linzhao Cheng; Holly Hammond; Zhaohui Ye; Xiangcan Zhan; Gautam Dravid
Journal:  Stem Cells       Date:  2003       Impact factor: 6.277
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3.  Establishment of porcine and human expanded potential stem cells.

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Journal:  Nat Cell Biol       Date:  2019-06-03       Impact factor: 28.824

4.  Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.

Authors:  Despina Soteriou; Banu Iskender; Adam Byron; Jonathan D Humphries; Simon Borg-Bartolo; Marie-Claire Haddock; Melissa A Baxter; David Knight; Martin J Humphries; Susan J Kimber
Journal:  J Biol Chem       Date:  2013-05-08       Impact factor: 5.157

5.  Robust method for TALEN-edited correction of pF508del in patient-specific induced pluripotent stem cells.

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6.  High quality clinical grade human embryonic stem cell lines derived from fresh discarded embryos.

Authors:  Jinpei Ye; Nicola Bates; Despina Soteriou; Lisa Grady; Clare Edmond; Alex Ross; Alan Kerby; Philip A Lewis; Tope Adeniyi; Ronnie Wright; Kay V Poulton; Marcus Lowe; Susan J Kimber; Daniel R Brison
Journal:  Stem Cell Res Ther       Date:  2017-06-05       Impact factor: 6.832

7.  Global gene expression profiling of individual human oocytes and embryos demonstrates heterogeneity in early development.

Authors:  Lisa Shaw; Sharon F Sneddon; Leo Zeef; Susan J Kimber; Daniel R Brison
Journal:  PLoS One       Date:  2013-05-22       Impact factor: 3.240

8.  Graphene Oxide promotes embryonic stem cell differentiation to haematopoietic lineage.

Authors:  Eva Garcia-Alegria; Maria Iliut; Monika Stefanska; Claudio Silva; Sebastian Heeg; Susan J Kimber; Valerie Kouskoff; Georges Lacaud; Aravind Vijayaraghavan; Kiran Batta
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  8 in total

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