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Literature DB >> 20172997

Interaction of an essential Escherichia coli GTPase, Der, with the 50S ribosome via the KH-like domain.

Abstract

Der, an essential Escherichia coli tandem GTPase, has been implicated in 50S subunit biogenesis. The rrmJ gene encodes a methyltransferase that modifies the U2552 residue of 23S rRNA, and its deletion causes a severe growth defect. Peculiarly, overexpression of Der suppresses growth impairment. In this study, using an rrmJ-deletion strain, we demonstrated that two GTPase domains of Der regulate its association with 50S subunit via the KH-like domain. We also identified a region of Der that is critical for its specific interaction with 50S subunit.

Entities: Disease Gene Species

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Year: 2010 PMID： 20172997 PMCID： PMC2849445 DOI： 10.1128/JB.00045-10

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

27 in total

1. Solution structure of the A loop of 23S ribosomal RNA.

Authors: S C Blanchard; J D Puglisi
Journal: Proc Natl Acad Sci U S A Date: 2001-03-20 Impact factor: 11.205

2. The FtsJ/RrmJ heat shock protein of Escherichia coli is a 23 S ribosomal RNA methyltransferase.

Authors: T Caldas; E Binet; P Bouloc; A Costa; J Desgres; G Richarme
Journal: J Biol Chem Date: 2000-06-02 Impact factor: 5.157

3. Translational defects of Escherichia coli mutants deficient in the Um(2552) 23S ribosomal RNA methyltransferase RrmJ/FTSJ.

Authors: T Caldas; E Binet; P Bouloc; G Richarme
Journal: Biochem Biophys Res Commun Date: 2000-05-19 Impact factor: 3.575

Interaction of an essential Escherichia coli GTPase, Der, with the 50S ribosome via the KH-like domain.

1. Solution structure of the A loop of 23S ribosomal RNA.

2. The FtsJ/RrmJ heat shock protein of Escherichia coli is a 23 S ribosomal RNA methyltransferase.

3. Translational defects of Escherichia coli mutants deficient in the Um(2552) 23S ribosomal RNA methyltransferase RrmJ/FTSJ.

Review 4. Evolution of a molecular switch: universal bacterial GTPases regulate ribosome function.

5. RNA methylation under heat shock control.

6. Domain arrangement of Der, a switch protein containing two GTPase domains.

7. The rlmB gene is essential for formation of Gm2251 in 23S rRNA but not for ribosome maturation in Escherichia coli.

8. An essential GTPase, der, containing double GTP-binding domains from Escherichia coli and Thermotoga maritima.

9. Overexpression of two different GTPases rescues a null mutation in a heat-induced rRNA methyltransferase.

10. Active site in RrmJ, a heat shock-induced methyltransferase.

1. Crippling the essential GTPase Der causes dependence on ribosomal protein L9.

Review 2. The universally conserved prokaryotic GTPases.

3. Heterologous Expression of Der Homologs in an Escherichia coli der Mutant and Their Functional Complementation.

4. Structural insights into the function of a unique tandem GTPase EngA in bacterial ribosome assembly.