Literature DB >> 2017189

Bone-inducing activity of mature BMP-2b produced from a hybrid BMP-2a/2b precursor.

R G Hammonds1, R Schwall, A Dudley, L Berkemeier, C Lai, J Lee, N Cunningham, A H Reddi, W I Wood, A J Mason.   

Abstract

The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2b has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo.

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Year:  1991        PMID： 2017189     DOI： 10.1210/mend-5-1-149

Source DB:  PubMed          Journal:  Mol Endocrinol        ISSN： 0888-8809


  25 in total

Review 1.  Soluble, insoluble and geometric signals sculpt the architecture of mineralized tissues.

Authors:  U Ripamonti
Journal:  J Cell Mol Med       Date:  2004 Apr-Jun       Impact factor: 5.310

2.  [Evaluation of the osteoinductive potential of genetically modified BMP-2 variants].

Authors:  K K Würzler; J Emmert; F Eichelsbacher; N R Kübler; W Sebald; J F Reuther
Journal:  Mund Kiefer Gesichtschir       Date:  2004-02-03

Review 3.  Structural Biology and Evolution of the TGF-β Family.

Authors:  Andrew P Hinck; Thomas D Mueller; Timothy A Springer
Journal:  Cold Spring Harb Perspect Biol       Date:  2016-12-01       Impact factor: 10.005

4.  Expression of active hBMP2 in transgenic tobacco plants.

Authors:  Guangli Suo; Bing Chen; Jingyu Zhang; Yuan Gao; Xia Wang; Zhengquan He; Jianwu Dai
Journal:  Plant Cell Rep       Date:  2006-07-04       Impact factor: 4.570

Review 5.  [Osteoinduction and -reparation].

Authors:  N R Kübler
Journal:  Mund Kiefer Gesichtschir       Date:  1997-02

6.  The prodomain of BMP4 is necessary and sufficient to generate stable BMP4/7 heterodimers with enhanced bioactivity in vivo.

Authors:  Judith M Neugebauer; Sunjong Kwon; Hyung-Seok Kim; Nathan Donley; Anup Tilak; Shailaja Sopory; Jan L Christian
Journal:  Proc Natl Acad Sci U S A       Date:  2015-04-20       Impact factor: 11.205

7.  Induction of bone formation by transforming growth factor-beta2 in the non-human primate Papio ursinus and its modulation by skeletal muscle responding stem cells.

Authors:  U Ripamonti; L C Roden
Journal:  Cell Prolif       Date:  2010-06       Impact factor: 6.831

8.  Bone formation in vitro and in nude mice by human osteosarcoma cells.

Authors:  A Ogose; T Motoyama; T Hotta; H Watanabe; H E Takahashi
Journal:  Virchows Arch       Date:  1995       Impact factor: 4.064

9.  Identification and characterization of cellular binding proteins (receptors) for recombinant human bone morphogenetic protein 2B, an initiator of bone differentiation cascade.

Authors:  V M Paralkar; R G Hammonds; A H Reddi
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

10.  Osteogenin and recombinant bone morphogenetic protein 2B are chemotactic for human monocytes and stimulate transforming growth factor beta 1 mRNA expression.

Authors:  N S Cunningham; V Paralkar; A H Reddi
Journal:  Proc Natl Acad Sci U S A       Date:  1992-12-15       Impact factor: 11.205
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