Literature DB >> 20171160

Activity of an enzyme immobilized on superparamagnetic particles in a rotational magnetic field.

Toru Mizuki1, Noriyuki Watanabe, Yutaka Nagaoka, Tadamasa Fukushima, Hisao Morimoto, Ron Usami, Toru Maekawa.   

Abstract

We immobilize alpha-amylase extracted from Bacillus Iicheniformis on the surfaces of superparamagnetic particles and investigate the effect of a rotational magnetic field on the enzyme's activity. We find that the activity of the enzyme molecules immobilized on superparamagnetic particles increases in the rotational magnetic field and reaches maximum at a certain frequency. We clarify the effect of the cluster structures formed by the superparamagnetic particles on the activity. Enzyme reactions are enhanced even in a tiny volume of solution using the present method, which is very important for the development of efficient micro reactors and micro total analysis systems (mu-TAS). Copyright 2010 Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 20171160     DOI： 10.1016/j.bbrc.2010.02.081

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

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Authors:  Toru Mizuki; Miyuki Sawai; Yutaka Nagaoka; Hisao Morimoto; Toru Maekawa
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4.  Encouragement of Enzyme Reaction Utilizing Heat Generation from Ferromagnetic Particles Subjected to an AC Magnetic Field.

Authors:  Masashi Suzuki; Atsushi Aki; Toru Mizuki; Toru Maekawa; Ron Usami; Hisao Morimoto
Journal:  PLoS One       Date:  2015-05-19       Impact factor: 3.240

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8.  Magneto-mechanical destruction of cancer-associated fibroblasts using ultra-small iron oxide nanoparticles and low frequency rotating magnetic fields.

Authors:  Sara Lopez; Nicolas Hallali; Yoann Lalatonne; Arnaud Hillion; Joana C Antunes; Nizar Serhan; Pascal Clerc; Daniel Fourmy; Laurence Motte; Julian Carrey; Véronique Gigoux
Journal:  Nanoscale Adv       Date:  2021-11-18

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Authors:  Zoheb Karim; Rohana Adnan; Mohd Saquib Ansari
Journal:  PLoS One       Date:  2012-07-27       Impact factor: 3.240
  9 in total

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