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Literature DB >> 2016777

High-efficiency formation of influenza virus transfectants.

Abstract

cDNA-derived RNAs were introduced into the genomes of influenza viruses by using an improved ribonucleoprotein (RNP) transfection protocol. Up to 10(5) viral transfectants with a novel neuraminidase gene could be obtained by using a 35-mm dish (10(6) cells) for RNP transfection. In addition to genes coding for surface proteins (hemagglutinin and neuraminidase), we also exchanged a gene coding for nonsurface proteins. The cDNA-derived influenza A/PR/8/34 virus NS gene was introduced into a temperature-sensitive mutant with a defect in this gene. We suggest that the term influenza virus transfectant be used for those viruses which are made by RNP transfection with cDNA-derived RNA.

Entities: Disease Species

Mesh：

Substances：

Year: 1991 PMID： 2016777 PMCID： PMC240634

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

9 in total

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9 in total

54 in total

1. Polyuridylated mRNA synthesized by a recombinant influenza virus is defective in nuclear export.

Authors: L L Poon; E Fodor; G G Brownlee
Journal: J Virol Date: 2000-01 Impact factor: 5.103

2. Influenza A virus RNA polymerase has the ability to stutter at the polyadenylation site of a viral RNA template during RNA replication.

Authors: H Zheng; H A Lee; P Palese; A García-Sastre
Journal: J Virol Date: 1999-06 Impact factor: 5.103

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Authors: X Wang; M Li; H Zheng; T Muster; P Palese; A A Beg; A García-Sastre
Journal: J Virol Date: 2000-12 Impact factor: 5.103

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Journal: J Virol Date: 1992-07 Impact factor: 5.103

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Journal: Proc Natl Acad Sci U S A Date: 1991-11-01 Impact factor: 11.205

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Authors: M Enami; K Enami
Journal: J Virol Date: 2000-06 Impact factor: 5.103

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Authors: M R Castrucci; Y Kawaoka
Journal: J Virol Date: 1995-05 Impact factor: 5.103