Literature DB >> 20164273

Comparison of traditional phenotypic identification methods with partial 5' 16S rRNA gene sequencing for species-level identification of nonfermenting Gram-negative bacilli.

Joann L Cloud1, Dag Harmsen, Peter C Iwen, James J Dunn, Gerri Hall, Paul Rocco Lasala, Karen Hoggan, Deborah Wilson, Gail L Woods, Alexander Mellmann.   

Abstract

Correct identification of nonfermenting Gram-negative bacilli (NFB) is crucial for patient management. We compared phenotypic identifications of 96 clinical NFB isolates with identifications obtained by 5' 16S rRNA gene sequencing. Sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify NFB.

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Year:  2010        PMID： 20164273      PMCID： PMC2849612          DOI： 10.1128/JCM.00169-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


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