OBJECTIVE: To study the differential patterns of gene expression in skeletal muscle and adipose tissue between type 2 diabetes mellitus (T2DM) patients and healthy subjects using DNA microarray analysis. METHODS: T2DM patiens were divided into female group, young male group and old male group. DNA microarray analysis and quantitative real-time PCR were carried out to analyze the relation between gene expressions and T2DM. RESULTS: The mRNA expression of 298, 578, and 350 genes was changed in the skeletal muscle of diabetes mellitus patients compared with control subjects. The 1320, 1143, and 2847 genes were modified in adipose tissue of the three groups. Among the genes surveyed, the change of 25 and 39 gene transcripts in skeletal muscle and adipose tissue was > or = 2 folds. These differentially expressed genes were classified into 15 categories according to their functions. CONCLUSION: New genes are found and T2DM can be prevented or cured.
OBJECTIVE: To study the differential patterns of gene expression in skeletal muscle and adipose tissue between type 2 diabetes mellitus (T2DM) patients and healthy subjects using DNA microarray analysis. METHODS: T2DM patiens were divided into female group, young male group and old male group. DNA microarray analysis and quantitative real-time PCR were carried out to analyze the relation between gene expressions and T2DM. RESULTS: The mRNA expression of 298, 578, and 350 genes was changed in the skeletal muscle of diabetes mellituspatients compared with control subjects. The 1320, 1143, and 2847 genes were modified in adipose tissue of the three groups. Among the genes surveyed, the change of 25 and 39 gene transcripts in skeletal muscle and adipose tissue was > or = 2 folds. These differentially expressed genes were classified into 15 categories according to their functions. CONCLUSION: New genes are found and T2DM can be prevented or cured.
Authors: Sander A J Verbrugge; Sebastian Gehlert; Lian E M Stadhouders; Daniel Jacko; Thorben Aussieker; Gerard M J de Wit; Ilse S P Vogel; Carla Offringa; Martin Schönfelder; Richard T Jaspers; Henning Wackerhage Journal: Int J Mol Sci Date: 2020-09-25 Impact factor: 5.923