Qing Wang1, Hequan Li, Zhiyuan Zhang, Yinan Yao, Jianying Zhou. 1. Department of Respiratory Diseases, The First Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou, People's Republic of China. shanhaiwq1983@yahoo.com.cn
Abstract
OBJECTIVE AND DESIGN: In this study, we explored whether prolonged ovalbumin (OVA) exposure in sensitized mice created an environment suitable for Th17 differentiation. MATERIALS AND METHODS: BALB/c and C57BL/6 mice (n = 36), after intraperitoneal OVA sensitization on days 0 and 12, received prolonged OVA aerosol challenges up to day 55. Airway inflammatory cell levels, cytokine profiles, and Th17 cell infiltration were evaluated after sacrifice. RESULTS: Prolonged OVA challenge caused inflammation that was characterized by raised influxes of airway macrophages and neutrophils. Following long-term exposure, Th17 cytokines and Th17 cell numbers progressively increased in the lung (P < 0.05) along with increased production of Th17 polarization-related factors, including TGF-beta, IL-6, and IL-23. The lineage-specific transcription factor for Th17 subsets, RORgammat, displayed similar upregulation throughout the OVA challenge (P < 0.05). CONCLUSIONS: Prolonged OVA challenge induces an environment that facilitates Th17 polarization.
OBJECTIVE AND DESIGN: In this study, we explored whether prolonged ovalbumin (OVA) exposure in sensitized mice created an environment suitable for Th17 differentiation. MATERIALS AND METHODS: BALB/c and C57BL/6 mice (n = 36), after intraperitoneal OVA sensitization on days 0 and 12, received prolonged OVA aerosol challenges up to day 55. Airway inflammatory cell levels, cytokine profiles, and Th17 cell infiltration were evaluated after sacrifice. RESULTS: Prolonged OVA challenge caused inflammation that was characterized by raised influxes of airway macrophages and neutrophils. Following long-term exposure, Th17 cytokines and Th17 cell numbers progressively increased in the lung (P < 0.05) along with increased production of Th17 polarization-related factors, including TGF-beta, IL-6, and IL-23. The lineage-specific transcription factor for Th17 subsets, RORgammat, displayed similar upregulation throughout the OVA challenge (P < 0.05). CONCLUSIONS: Prolonged OVA challenge induces an environment that facilitates Th17 polarization.
Authors: Estelle Bettelli; Yijun Carrier; Wenda Gao; Thomas Korn; Terry B Strom; Mohamed Oukka; Howard L Weiner; Vijay K Kuchroo Journal: Nature Date: 2006-04-30 Impact factor: 49.962
Authors: Ivaylo I Ivanov; Brent S McKenzie; Liang Zhou; Carlos E Tadokoro; Alice Lepelley; Juan J Lafaille; Daniel J Cua; Dan R Littman Journal: Cell Date: 2006-09-22 Impact factor: 41.582
Authors: Filip K Swirski; Dusan Sajic; Clinton S Robbins; Beata U Gajewska; Manel Jordana; Martin R Stämpfli Journal: J Immunol Date: 2002-10-01 Impact factor: 5.422
Authors: Lucila G G Pacífico; Fábio A V Marinho; Cristina T Fonseca; Michele M Barsante; Vanessa Pinho; Policarpo A Sales-Junior; Luciana S Cardoso; Maria Ilma Araújo; Edgar M Carvalho; Geovanni D Cassali; Mauro M Teixeira; Sergio C Oliveira Journal: Infect Immun Date: 2008-09-29 Impact factor: 3.441