Human ovarian tissue cryopreservation: quality of follicles as a criteria of effectiveness.

Experiments comparing vitrification and conventional freezing of mammalian ovarian tissue show that vitrification can also guarantee the storage of viable follicles after warming, but conventional freezing is more effective. The central goal of cryotechnology is the preservation of intact follicles. This article presents a critical opinion about the normality of follicles after vitrification of human ovarian tissue and microbial contamination as a result of direct contact of this tissue with liquid nitrogen at vitrification. Copyright (c) 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.