Replication of poliovirus (LSc 2 ab) in perfused cultures of MRC-5 diploid cells.

Application of a new perfusion culture system to the propagation of poliovirus type 1 (LSc 2 ab) in MRC-5 cells is described. Virus production was compared in perfused and stationary cultures of cells at the 26th to 30th population doubling. Yield of virus per cell and unit growth surface area were found to be consistently higher in the perfused system by factors of three to five fold respectively. The influence of experimental conditions on these results and the potential of perfusion for large scale vaccine manufacture are discussed.