Xuecai Ge1, Christopher L Frank, Froylan Calderon de Anda, Li-Huei Tsai. 1. Picower Institute for Learning and Memory, Department of Brain and Cognitive, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Building 46, Room 4235A, Cambridge, MA 02139, USA.
Abstract
Centrosome functions are important in multiple brain developmental processes. Proper functioning of the centrosome relies on assembly of protein components into the pericentriolar material. This dynamic assembly is mediated by the trafficking of pericentriolar satellites, which are comprised of centrosomal proteins. Here we demonstrate that trafficking of pericentriolar satellites requires the interaction between Hook3 and Pericentriolar Material 1 (PCM1). Hook3, previously shown to link the centrosome and the nucleus in C. elegans, is recruited to pericentriolar satellites through interaction with PCM1, a protein associated with schizophrenia. Disruption of the Hook3-PCM1 interaction in vivo impairs interkinetic nuclear migration, a featured behavior of embryonic neural progenitors. This in turn leads to overproduction of neurons and premature depletion of the neural progenitor pool in the developing neocortex. These results underscore the importance of centrosomal assembly in neurogenesis and provide potential insights into the etiology of brain developmental diseases related to the centrosome dysfunction. Copyright 2010 Elsevier Inc. All rights reserved.
Centrosome functions are important in multiple brain developmental processes. Proper functioning of the centrosome relies on assembly of protein compn>onents into the pericentriolar material. This dynamic assembly is mediated by the trafficking of pericentriolar satellites, which are compn>rised of centrosomal proteins. Here we demonstrate that trafficking of pericentriolar satellites requires the interaction between n>an class="Gene">Hook3 and Pericentriolar Material 1 (PCM1). Hook3, previously shown to link the centrosome and the nucleus in C. elegans, is recruited to pericentriolar satellites through interaction with PCM1, a protein associated with schizophrenia. Disruption of the Hook3-PCM1 interaction in vivo impairs interkinetic nuclear migration, a featured behavior of embryonic neural progenitors. This in turn leads to overproduction of neurons and premature depletion of the neural progenitor pool in the developing neocortex. These results underscore the importance of centrosomal assembly in neurogenesis and provide potential insights into the etiology of brain developmental diseases related to the centrosome dysfunction. Copyright 2010 Elsevier Inc. All rights reserved.
Authors: S R Datta; A McQuillin; M Rizig; E Blaveri; S Thirumalai; G Kalsi; J Lawrence; N J Bass; V Puri; K Choudhury; J Pimm; C Crombie; G Fraser; N Walker; D Curtis; M Zvelebil; A Pereira; R Kandaswamy; D St Clair; H M D Gurling Journal: Mol Psychiatry Date: 2008-12-02 Impact factor: 15.992
Authors: Mei-Chong Wendy Lee; Fernando J Lopez-Diaz; Shahid Yar Khan; Muhammad Akram Tariq; Yelena Dayn; Charles Joseph Vaske; Amie J Radenbaugh; Hyunsung John Kim; Beverly M Emerson; Nader Pourmand Journal: Proc Natl Acad Sci U S A Date: 2014-10-22 Impact factor: 11.205