PURPOSE: To assess the expression of Epiplakin1 (Eppk1) in the developing and adult mouse retina. METHODS: Eppk1 expression was examined using reverse transcription-polymerase chain reaction (RTPCR) analysis and immunoblot analysis. The Eppk1 expression pattern was examined by immunohistochemical analysis in embryonic and adult C57BL/6J mice. RESULTS: Both Eppk1 mRNA and protein were detected in adult mouse retina. Eppk1 was expressed in nestin-positive neural progenitor cells in the embryonic retina. In adults, Eppk1 was expressed in retinal ganglion cells, bipolar cells, and Müller glial cells. CONCLUSIONS: These results suggest that Eppk1 is expressed both in embryonic and adult retina and may be involved in retinal development.
PURPOSE: To assess the expression of Epiplakin1 (Eppk1) in the developing and adult mouse retina. METHODS:Eppk1 expression was examined using reverse transcription-polymerase chain reaction (RTPCR) analysis and immunoblot analysis. The Eppk1 expression pattern was examined by immunohistochemical analysis in embryonic and adult C57BL/6J mice. RESULTS: Both Eppk1 mRNA and protein were detected in adult mouse retina. Eppk1 was expressed in nestin-positive neural progenitor cells in the embryonic retina. In adults, Eppk1 was expressed in retinal ganglion cells, bipolar cells, and Müller glial cells. CONCLUSIONS: These results suggest that Eppk1 is expressed both in embryonic and adult retina and may be involved in retinal development.
Authors: S Fujiwara; N Takeo; Y Otani; D A Parry; M Kunimatsu; R Lu; M Sasaki; N Matsuo; M Khaleduzzaman; H Yoshioka Journal: J Biol Chem Date: 2001-01-17 Impact factor: 5.157
Authors: Ani V Das; Kavita B Mallya; Xing Zhao; Faraz Ahmad; Sumitra Bhattacharya; Wallace B Thoreson; Ganapati V Hegde; Iqbal Ahmad Journal: Dev Biol Date: 2006-07-29 Impact factor: 3.582
Authors: Mike O Karl; Susan Hayes; Branden R Nelson; Kristine Tan; Brian Buckingham; Thomas A Reh Journal: Proc Natl Acad Sci U S A Date: 2008-11-25 Impact factor: 11.205