BACKGROUND: Disulfide-linked oligodeoxyribonucleotide (ODN) liposomes were formulated and evaluated for the delivery of antisense ODN G3139 in KB human oral carcinoma cells. MATERIALS AND METHODS: Liposomes composed of 1,2-di-(9Z-octadecenoyl)-3-trimethylammo-nium-propane (DOTAP)/egg phosphatidylcholine/alpha-tocopheryl polyethylene glycol 1000 succinate were incorporated with hydrophobized disulfide-linked ODN. Disulfide-linked ODN liposomes were characterized for their size, ODN intracellular delivery, Bcl-2 mRNA and protein expression, growth inhibition, and chemosensitization. RESULTS: Intracellular delivery of ODN with disulfide-linked ODN liposomes was more efficient than that with non-liposomal hydrophobized disulfide-linked ODN. Treatment of the cells with disulfide-linked ODN liposomes resulted in efficient Bcl-2 down-regulation greater than that with hydrophobized disulfide-linked ODN and consistent with that of cellular growth inhibition and the sensitization to daunorubicin in KB cells. Disulfide-linked ODN liposomes exhibited superior colloidal stability during 5-week storage. CONCLUSION: Disulfide-linked liposomes are effective delivery vehicles for antisense ODN.
BACKGROUND:Disulfide-linked oligodeoxyribonucleotide (ODN) liposomes were formulated and evaluated for the delivery of antisense ODNG3139 in KB humanoral carcinoma cells. MATERIALS AND METHODS: Liposomes composed of 1,2-di-(9Z-octadecenoyl)-3-trimethylammo-nium-propane (DOTAP)/egg phosphatidylcholine/alpha-tocopheryl polyethylene glycol 1000 succinate were incorporated with hydrophobized disulfide-linked ODN. Disulfide-linked ODN liposomes were characterized for their size, ODN intracellular delivery, Bcl-2 mRNA and protein expression, growth inhibition, and chemosensitization. RESULTS: Intracellular delivery of ODN with disulfide-linked ODN liposomes was more efficient than that with non-liposomal hydrophobized disulfide-linked ODN. Treatment of the cells with disulfide-linked ODN liposomes resulted in efficient Bcl-2 down-regulation greater than that with hydrophobized disulfide-linked ODN and consistent with that of cellular growth inhibition and the sensitization to daunorubicin in KB cells. Disulfide-linked ODN liposomes exhibited superior colloidal stability during 5-week storage. CONCLUSION:Disulfide-linked liposomes are effective delivery vehicles for antisense ODN.
Authors: N Maurer; K F Wong; H Stark; L Louie; D McIntosh; T Wong; P Scherrer; S C Semple; P R Cullis Journal: Biophys J Date: 2001-05 Impact factor: 4.033
Authors: Olga B Garbuzenko; Maha Saad; Seema Betigeri; Min Zhang; Alexandre A Vetcher; Viatcheslav A Soldatenkov; David C Reimer; Vitaly P Pozharov; Tamara Minko Journal: Pharm Res Date: 2008-10-29 Impact factor: 4.200
Authors: Xulang Zhang; Chee Guan Koh; Bo Yu; Shujun Liu; Longzhu Piao; Guido Marcucci; Robert J Lee; L James Lee Journal: Pharm Res Date: 2009-03-17 Impact factor: 4.200