AIMS: Micro-ribonucleic acid (miRNA) are noncoding RNA molecules of 21 to 24 nt that regulate the expression of target genes in a post-transcriptional manner. Evidence indicates that miRNA play essential roles in embryogenesis, cell differentiation and pathogenesis of human diseases. This study describes a comparison between the microRNA profile of human-papillomavirus-negative cervical squamous cell carcinoma patients and controls, in order to develop further understanding of the pathogenesis of cervical squamous cell carcinomas. METHODS: MiRNA were isolated from tumor tissues of five human-papillomavirus-negative cervical squamous cell carcinoma patients and five healthy controls in order to perform miRNA microarray chip analysis. The chip results were then confirmed by northern blot analysis. RESULTS: A total of 27 miRNA differentially expressed between the squamous cell carcinoma patients and the healthy controls were identified. CONCLUSION: This work indicates that these miRNA may be potential diagnosis biomarkers and probable factors involved in the pathogenesis of cervical squamous cell carcinomas.
AIMS: Micro-ribonucleic acid (miRNA) are noncoding RNA molecules of 21 to 24 nt that regulate the expression of target genes in a post-transcriptional manner. Evidence indicates that miRNA play essential roles in embryogenesis, cell differentiation and pathogenesis of human diseases. This study describes a comparison between the microRNA profile of human-papillomavirus-negative cervical squamous cell carcinomapatients and controls, in order to develop further understanding of the pathogenesis of cervical squamous cell carcinomas. METHODS: MiRNA were isolated from tumor tissues of five human-papillomavirus-negative cervical squamous cell carcinomapatients and five healthy controls in order to perform miRNA microarray chip analysis. The chip results were then confirmed by northern blot analysis. RESULTS: A total of 27 miRNA differentially expressed between the squamous cell carcinomapatients and the healthy controls were identified. CONCLUSION: This work indicates that these miRNA may be potential diagnosis biomarkers and probable factors involved in the pathogenesis of cervical squamous cell carcinomas.
Authors: Alison P Sanders; Chris Gennings; Katherine Svensson; Valeria Motta; Adriana Mercado-Garcia; Maritsa Solano; Andrea A Baccarelli; Martha M Tellez-Rojo; Robert O Wright; Heather H Burris Journal: Epigenomics Date: 2016-12-12 Impact factor: 4.778
Authors: Aaron E Hoffman; Ran Liu; Alan Fu; Tongzhang Zheng; Frank Slack; Yong Zhu Journal: Cancer Epidemiol Biomarkers Prev Date: 2013-01-18 Impact factor: 4.254
Authors: Bin Zhang; Jun Chen; Zhenghua Ren; Yongbin Chen; Jinhui Li; Xia Miao; Yang Song; Tao Zhao; Yurong Li; Yongquan Shi; Dongqing Ren; Junye Liu Journal: Cancer Cell Int Date: 2013-11-27 Impact factor: 5.722