| Literature DB >> 20148974 |
Chenyang Huang1, Jingyu Xu, Wei Gao, Qiang Chen, Hexiang Wang, Jinxia Zhang.
Abstract
To examine why we failed in direct sequencing of rRNA gene internal transcribed spacer (ITS) in Pleurotus nebrodensis, we obtained monokaryons of P. nebrodensis (00489 and 00491) using a protoplast monokaryonization technique. PCR products of ITS amplifications were sequenced. There was a base pair insertion/deletion difference between the two nuclei of P. nebrodensis that led to failure in direct sequencing.Entities:
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Year: 2010 PMID： 20148974 DOI： 10.1111/j.1574-6968.2010.01891.x
Source DB: PubMed Journal: FEMS Microbiol Lett ISSN： 0378-1097 Impact factor: 2.742