Literature DB >> 20147141

Quick preparation of genomic DNA from Drosophila.

Audrey M Huang, E Jay Rehm, Gerald M Rubin.   

Abstract

This protocol describes a standard miniprep for Drosophila melanogaster that requires very few flies and produces high-quality DNA. This method can also be used to isolate RNA when RNase-free conditions are utilized; an extra step must be taken to rid the sample of genomic DNA (e.g., RNase-free DNase digestion).

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Year:  2009        PMID: 20147141     DOI: 10.1101/pdb.prot5198

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN: 1559-6095


  17 in total

1.  Identification of RNA-binding protein targets with HyperTRIBE.

Authors:  Reazur Rahman; Weijin Xu; Hua Jin; Michael Rosbash
Journal:  Nat Protoc       Date:  2018-08       Impact factor: 13.491

2.  Decapentaplegic and growth control in the developing Drosophila wing.

Authors:  Takuya Akiyama; Matthew C Gibson
Journal:  Nature       Date:  2015-11-09       Impact factor: 49.962

3.  Mediation of Drosophila autosomal dosage effects and compensation by network interactions.

Authors:  John H Malone; Dong-Yeon Cho; Nicolas R Mattiuzzo; Carlo G Artieri; Lichun Jiang; Ryan K Dale; Harold E Smith; Jennifer McDaniel; Sarah Munro; Marc Salit; Justen Andrews; Teresa M Przytycka; Brian Oliver
Journal:  Genome Biol       Date:  2012-04-24       Impact factor: 13.583

4.  Testes-specific hemoglobins in Drosophila evolved by a combination of sub- and neofunctionalization after gene duplication.

Authors:  Eva Gleixner; Holger Herlyn; Stefan Zimmerling; Thorsten Burmester; Thomas Hankeln
Journal:  BMC Evol Biol       Date:  2012-03-19       Impact factor: 3.260

5.  Combinatorial effects of transposable elements on gene expression and phenotypic robustness in Drosophila melanogaster development.

Authors:  Alexa W Clemmons; Steven A Wasserman
Journal:  G3 (Bethesda)       Date:  2013-09-04       Impact factor: 3.154

6.  Empirical validation of pooled whole genome population re-sequencing in Drosophila melanogaster.

Authors:  Yuan Zhu; Alan O Bergland; Josefa González; Dmitri A Petrov
Journal:  PLoS One       Date:  2012-07-26       Impact factor: 3.240

7.  Newly Characterized Region of CP190 Associates with Microtubules and Mediates Proper Spindle Morphology in Drosophila Stem Cells.

Authors:  Karen M Plevock; Brian J Galletta; Kevin C Slep; Nasser M Rusan
Journal:  PLoS One       Date:  2015-12-09       Impact factor: 3.240

8.  Rapid sequential spread of two Wolbachia variants in Drosophila simulans.

Authors:  Peter Kriesner; Ary A Hoffmann; Siu F Lee; Michael Turelli; Andrew R Weeks
Journal:  PLoS Pathog       Date:  2013-09-12       Impact factor: 6.823

9.  A Genome-Wide Screen for Dendritically Localized RNAs Identifies Genes Required for Dendrite Morphogenesis.

Authors:  Mala Misra; Hendia Edmund; Darragh Ennis; Marissa A Schlueter; Jessica E Marot; Janet Tambasco; Ida Barlow; Sara Sigurbjornsdottir; Renjith Mathew; Ana Maria Vallés; Waldemar Wojciech; Siegfried Roth; Ilan Davis; Maria Leptin; Elizabeth R Gavis
Journal:  G3 (Bethesda)       Date:  2016-08-09       Impact factor: 3.154

10.  Expression of the Troponin C at 41C Gene in Adult Drosophila Tubular Muscles Depends upon Both Positive and Negative Regulatory Inputs.

Authors:  Maria B Chechenova; Sara Maes; Richard M Cripps
Journal:  PLoS One       Date:  2015-12-07       Impact factor: 3.240

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