Jing Wang1, Wei-Hong Wang, Jiang Li, Fang-Xun Liu. 1. Department of Gastroenterology, Peking University First Hospital, 8 Xishiku Avenue, Xicheng District, Beijing 100034, China.
Abstract
AIM: To investigate the effect of celecoxib, a selective COX-2 inhibitor, on Helicobacter pylori (H. pylori) colonization-related factors and its mechanism. METHODS: After co-incubation with celecoxib, morphology of H. pylori strain 26695 was observed under a transmission electron microscope. Flagella motility was assessed by stab agar motility test. Adherence of H. pylori to AGS cells was determined by enzyme linked immunosorbent assay. Levels of mRNA expression in flagellar genes (flaA, flaB), urease genes (ureA, ureB) and adhesin genes (babA, sabA, alpA, alpB, hpaA, hopZ) were measured by real-time polymerase chain reaction. RESULTS: Separation and non-integrity of bacterial cell wall, rarefaction and asymmetry of cytoplasm, and even lysis of H. pylori were observed in the presence of celecoxib. When H. pylori strains were incubated in the presence of celecoxib, their flagellar motility and adherence to AGS cells were inhibited. The expression of ureA, ureB, babA, sabA, alpA, alpB, hpaA, hopZ was up-regulated while the expression of flaA, flaB was down-regulated in the presence of celecoxib. CONCLUSION: Celecoxib inhibits flagellar motility and adherence of H. pylori to AGS cells, and destructs their normal structure in vitro.
AIM: To investigate the effect of celecoxib, a selective COX-2 inhibitor, on Helicobacter pylori (H. pylori) colonization-related factors and its mechanism. METHODS: After co-incubation with celecoxib, morphology of H. pylori strain 26695 was observed under a transmission electron microscope. Flagella motility was assessed by stab agar motility test. Adherence of H. pylori to AGS cells was determined by enzyme linked immunosorbent assay. Levels of mRNA expression in flagellar genes (flaA, flaB), urease genes (ureA, ureB) and adhesin genes (babA, sabA, alpA, alpB, hpaA, hopZ) were measured by real-time polymerase chain reaction. RESULTS: Separation and non-integrity of bacterial cell wall, rarefaction and asymmetry of cytoplasm, and even lysis of H. pylori were observed in the presence of celecoxib. When H. pylori strains were incubated in the presence of celecoxib, their flagellar motility and adherence to AGS cells were inhibited. The expression of ureA, ureB, babA, sabA, alpA, alpB, hpaA, hopZ was up-regulated while the expression of flaA, flaB was down-regulated in the presence of celecoxib. CONCLUSION:Celecoxib inhibits flagellar motility and adherence of H. pylori to AGS cells, and destructs their normal structure in vitro.
Authors: Francis K L Chan; K F To; Justin C Y Wu; M Y Yung; W K Leung; Timothy Kwok; Y Hui; Henry L Y Chan; Cynthia S Y Chan; Elsie Hui; Jean Woo; Joseph J Y Sung Journal: Lancet Date: 2002-01-05 Impact factor: 79.321
Authors: Haim Shirin; Steven F Moss; Sandy Kancherla; Kevin Kancherla; Peter R Holt; I Bernard Weinstein; Emilia M Sordillo Journal: J Gastroenterol Hepatol Date: 2006-09 Impact factor: 4.029
Authors: Wei Hong Wang; Jia Qing Huang; Ge Fan Zheng; Shiu Kum Lam; Johan Karlberg; Benjamin Chun-Yu Wong Journal: J Natl Cancer Inst Date: 2003-12-03 Impact factor: 13.506
Authors: Q Gu; H H-X Xia; W H Wang; J D Wang; W M Wong; A O O Chan; M F Yuen; S K Lam; H K-L Cheung; X G Liu; B C-Y Wong Journal: Aliment Pharmacol Ther Date: 2004-09-15 Impact factor: 8.171
Authors: Rafaelle Fonseca de Matos; Lúcia Carla Vasconcelos Mendonça; Kaira Geiliane da Silva Souza; Adriana Aparecida Durães Fonseca; Elaine Mirla Souza Costa; Marcus Vinicius Dias de Lima; José Maria Dos Santos Vieira; Mioni Thielli Figueiredo Magalhães de Brito; Marta Chagas Monteiro Journal: Folia Microbiol (Praha) Date: 2016-11-19 Impact factor: 2.099
Authors: Nagendran Tharmalingam; Sa-Hyun Kim; Min Park; Hyun Jun Woo; Hyun Woo Kim; Ji Yeong Yang; Ki-Jong Rhee; Jong Bae Kim Journal: Infect Agent Cancer Date: 2014-12-16 Impact factor: 2.965