Literature DB >> 20143313

Monocyte and neutrophil isolation and migration assays.

Simon Yona1, Richard Hayhoe, Inbal Avraham-Davidi.   

Abstract

This unit describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell migration and polarization. The method employed here for the isolation of naïve phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring the modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Finally, a method is provided for imaging polarized cells by confocal microscopy.

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Year:  2010        PMID： 20143313     DOI： 10.1002/0471142735.im1415s88

Source DB:  PubMed          Journal:  Curr Protoc Immunol        ISSN： 1934-3671


  7 in total

1.  Methods of cell purification: a critical juncture for laboratory research and translational science.

Authors:  Peter J Amos; Esra Cagavi Bozkulak; Yibing Qyang
Journal:  Cells Tissues Organs       Date:  2011-10-12       Impact factor: 2.481

2.  Fate mapping reveals origins and dynamics of monocytes and tissue macrophages under homeostasis.

Authors:  Simon Yona; Ki-Wook Kim; Yochai Wolf; Alexander Mildner; Diana Varol; Michal Breker; Dalit Strauss-Ayali; Sergey Viukov; Martin Guilliams; Alexander Misharin; David A Hume; Harris Perlman; Bernard Malissen; Elazar Zelzer; Steffen Jung
Journal:  Immunity       Date:  2012-12-27       Impact factor: 31.745

3.  Bioinspired microfluidic assay for in vitro modeling of leukocyte-endothelium interactions.

Authors:  Giuseppina Lamberti; Balabhaskar Prabhakarpandian; Charles Garson; Ashley Smith; Kapil Pant; Bin Wang; Mohammad F Kiani
Journal:  Anal Chem       Date:  2014-08-05       Impact factor: 6.986

4.  Identification of Two Novel Compound Heterozygous PTPRQ Mutations Associated with Autosomal Recessive Hearing Loss in a Chinese Family.

Authors:  Xue Gao; Yu Su; Yu-Lan Chen; Ming-Yu Han; Yong-Yi Yuan; Jin-Cao Xu; Feng Xin; Mei-Guang Zhang; Sha-Sha Huang; Guo-Jian Wang; Dong-Yang Kang; Li-Ping Guan; Jian-Guo Zhang; Pu Dai
Journal:  PLoS One       Date:  2015-04-28       Impact factor: 3.240

5.  On-site education of VEGF-recruited monocytes improves their performance as angiogenic and arteriogenic accessory cells.

Authors:  Inbal Avraham-Davidi; Simon Yona; Myriam Grunewald; Limor Landsman; Clement Cochain; Jean Sebastien Silvestre; Haim Mizrahi; Mohammad Faroja; Dalit Strauss-Ayali; Matthias Mack; Steffen Jung; Eli Keshet
Journal:  J Exp Med       Date:  2013-10-28       Impact factor: 14.307

6.  Effects of Keratinocyte-Derived Cytokine (CXCL-1) on the Development of Theiler's Virus-Induced Demyelinating Disease.

Authors:  Min H Kang; Young H Jin; Byung S Kim
Journal:  Front Cell Infect Microbiol       Date:  2018-01-23       Impact factor: 5.293

7.  Ex vivo Pretreatment of Islets with Mitomycin C: Reduction in Immunogenic Potential of Islets by Suppressing Secretion of Multiple Chemotactic Factors.

Authors:  Naoya Sato; Junichiro Haga; Takayuki Anazawa; Akira Kenjo; Takashi Kimura; Ikuo Wada; Tsutomu Mori; Shigeru Marubashi; Mitsukazu Gotoh
Journal:  Cell Transplant       Date:  2017-08       Impact factor: 4.064
  7 in total

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