| Literature DB >> 20141937 |
Matilde Maqueda1, Emiliano Zamora, Nieves Rodríguez-Cousiño, Manuel Ramírez.
Abstract
Quick and accurate methods are required for the identification of industrial, environmental, and clinical yeast strains. We propose a rapid method for the simultaneous extraction of yeast mtDNA, nuclear DNA, and virus dsRNA. It is simpler, cheaper, and faster than the previously reported methods. It allows one to choose among a broad range of molecular analysis approaches for yeast typing, avoiding the need to use of several different methods for the separate extraction of each nucleic acid type. The application of this method followed by the combined analysis of mtDNA and dsRNA (ScV-M and W) is a highly attractive option for fast and efficient wine yeast typing.Entities:
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Year: 2009 PMID： 20141937 DOI： 10.1016/j.fm.2009.10.004
Source DB: PubMed Journal: Food Microbiol ISSN： 0740-0020 Impact factor: 5.516