Na An1, Xiang-ying Ou-yang, Wei Han. 1. Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Abstract
OBJECTIVE: To detect periodontopathic bacterial DNA in atherosclerotic vascular lesions in a group of Chinese patients by using polymerase chain reaction (PCR) techniques. METHODS: Ten human specimens of atherosclerotic plaque were obtained sterilely. The sclerotic lesions were blade into fine pieces and DNA was isolated from the samples. To detect Porphyromonas gingivalis(Pg), Tannerella forsythia(Tf), Aggregatibacter actinomycetemcomitans(Aa), Prevotella intermedia(Pi), Prevotella nigrescens(Pn), Treponema denticola(Td), Campylobacter rectus(Cr), PCR amplification of bacterial 16S ribosomal DNA (rDNA) was carried out. RESULTS: PCR assays for bacterial 16S rDNA indicated the presence of periodontal pathogens in 3 out of 10 surgical specimens. DNA of Pg were found in 3 samples, Tf was found only in one among the 3 samples and Pn was found in another sample among the 3 samples. CONCLUSION: The data confirm that DNA of periodontal pathogens present in atherosclerotic plaques. Pg, Tf, Pn may play a role in the development and progression of atherosclerosis in these Chinese patients. Further studies with large size samples are needed.
OBJECTIVE: To detect periodontopathic bacterial DNA in atherosclerotic vascular lesions in a group of Chinese patients by using polymerase chain reaction (PCR) techniques. METHODS: Ten human specimens of atherosclerotic plaque were obtained sterilely. The sclerotic lesions were blade into fine pieces and DNA was isolated from the samples. To detect Porphyromonas gingivalis(Pg), Tannerella forsythia(Tf), Aggregatibacter actinomycetemcomitans(Aa), Prevotella intermedia(Pi), Prevotella nigrescens(Pn), Treponema denticola(Td), Campylobacter rectus(Cr), PCR amplification of bacterial 16S ribosomal DNA (rDNA) was carried out. RESULTS: PCR assays for bacterial 16S rDNA indicated the presence of periodontal pathogens in 3 out of 10 surgical specimens. DNA of Pg were found in 3 samples, Tf was found only in one among the 3 samples and Pn was found in another sample among the 3 samples. CONCLUSION: The data confirm that DNA of periodontal pathogens present in atherosclerotic plaques. Pg, Tf, Pn may play a role in the development and progression of atherosclerosis in these Chinese patients. Further studies with large size samples are needed.