Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A nonviral minicircle vector for deriving human iPS cells.

Literature DB >> 20139967

A nonviral minicircle vector for deriving human iPS cells.

Fangjun Jia¹, Kitchener D Wilson, Ning Sun, Deepak M Gupta, Mei Huang, Zongjin Li, Nicholas J Panetta, Zhi Ying Chen, Robert C Robbins, Mark A Kay, Michael T Longaker, Joseph C Wu.

Abstract

Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Substances：
DNA, Circular

Year: 2010 PMID： 20139967 PMCID： PMC2892897 DOI： 10.1038/nmeth.1426

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

17 in total

1. Improved production and purification of minicircle DNA vector free of plasmid bacterial sequences and capable of persistent transgene expression in vivo.

Authors: Zhi-Ying Chen; Cheng-Yi He; Mark A Kay
Journal: Hum Gene Ther Date: 2005-01 Impact factor: 5.695

Review 2. Fat tissue: an underappreciated source of stem cells for biotechnology.

Authors: John K Fraser; Isabella Wulur; Zeni Alfonso; Marc H Hedrick
Journal: Trends Biotechnol Date: 2006-02-20 Impact factor: 19.536

3. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution.

Authors: Nimet Maherali; Rupa Sridharan; Wei Xie; Jochen Utikal; Sarah Eminli; Katrin Arnold; Matthias Stadtfeld; Robin Yachechko; Jason Tchieu; Rudolf Jaenisch; Kathrin Plath; Konrad Hochedlinger
Journal: Cell Stem Cell Date: 2007-06-07 Impact factor: 24.633

Review 10. Potential Strategies to Address the Major Clinical Barriers Facing Stem Cell Regenerative Therapy for Cardiovascular Disease: A Review.

Authors: Patricia K Nguyen; Evgenios Neofytou; June-Wha Rhee; Joseph C Wu
Journal: JAMA Cardiol Date: 2016-11-01 Impact factor: 14.676

A nonviral minicircle vector for deriving human iPS cells.

1. Improved production and purification of minicircle DNA vector free of plasmid bacterial sequences and capable of persistent transgene expression in vivo.

Review 2. Fat tissue: an underappreciated source of stem cells for biotechnology.

3. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution.

4. Induced pluripotent stem cells generated without viral integration.

5. Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence.

6. Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins.

7. Generation of germline-competent induced pluripotent stem cells.

8. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state.

9. Human CD4(+) T lymphocytes consistently respond to the latent Epstein-Barr virus nuclear antigen EBNA1.

10. Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein.

1. Effects of ionizing radiation on self-renewal and pluripotency of human embryonic stem cells.

2. Hepatitis C virus-induced cancer stem cell-like signatures in cell culture and murine tumor xenografts.

Review 3. Induced pluripotent stem cells--opportunities for disease modelling and drug discovery.

Review 4. Mechanism and methods to induce pluripotency.

5. Non-viral iPSCs: a safe way for therapy?

6. Methods of cell purification: a critical juncture for laboratory research and translational science.

7. Craniofacial reconstruction with induced pluripotent stem cells.

Review 8. Embryonic and induced pluripotent stem cells as a model for liver disease.

Review 9. Induced pluripotency: history, mechanisms, and applications.

Review 10. Potential Strategies to Address the Major Clinical Barriers Facing Stem Cell Regenerative Therapy for Cardiovascular Disease: A Review.