INTRODUCTION: The hypothesis that brief intermittent exercise-induced increases in human skeletal muscle metabolic mRNA is dependent on relative workload was investigated. METHODS:Trained (n = 10) and untrained (n = 8) subjects performed exhaustive intermittent cycling exercise (4 x 4 min at 85% of VO(2peak), interspersed by 3 min). Trained subjects also performed the intermittent exercise at the same absolute workload as the untrained subjects, corresponding to 70% of VO(2peak) (n = 6). RESULTS: Exercise at 85% of V(O2peak) elevated (P < 0.001) venous plasma lactate to 10.1 +/- 0.4 and 10.8 +/- 0.5 mM in the trained and untrained subjects, respectively. Peroxisome proliferator-activated receptor gamma coactivator 1alpha (PGC-1alpha) mRNAexpression was increased (P < 0.001) approximately four- to fivefold for several hours after exercise in both groups. After exercise at 70% of VO(2peak), venous plasma lactate was less (P < 0.001) elevated (3.1 +/- 0.7 mM) and PGC-1alpha mRNA content was less (P < 0.05) increased (approximately threefold) than after exercise at 85% of VO(2peak). Likewise, pyruvate dehydrogenase kinase 4 and hexokinaseII mRNA expressions were increased (P < 0.05) only after exercise performed at 85% of VO(2peak) in the trained subjects. Hypoxia-inducible factor2alphamRNA only increased (P < 0.05) 3 h into recovery in trained subjects, with no difference between the 70% and 85% of VO(2peak) trial. No change in hypoxia-inducible factor 1alpha, phosphofructokinase, citrate synthase, or lactate dehydrogenase, heart and muscle isoforms, mRNA expressions was detected after any of the exercise trials. CONCLUSIONS: The relative intensity of brief intermittent exercise is of major importance for the exercise-induced increase of several mRNA, including PGC-1alpha.
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INTRODUCTION: The hypothesis that brief intermittent exercise-induced increases in human skeletal muscle metabolic mRNA is dependent on relative workload was investigated. METHODS: Trained (n = 10) and untrained (n = 8) subjects performed exhaustive intermittent cycling exercise (4 x 4 min at 85% of VO(2peak), interspersed by 3 min). Trained subjects also performed the intermittent exercise at the same absolute workload as the untrained subjects, corresponding to 70% of VO(2peak) (n = 6). RESULTS: Exercise at 85% of V(O2peak) elevated (P < 0.001) venous plasma lactate to 10.1 +/- 0.4 and 10.8 +/- 0.5 mM in the trained and untrained subjects, respectively. Peroxisome proliferator-activated receptor gamma coactivator 1alpha (PGC-1alpha) mRNA expression was increased (P < 0.001) approximately four- to fivefold for several hours after exercise in both groups. After exercise at 70% of VO(2peak), venous plasma lactate was less (P < 0.001) elevated (3.1 +/- 0.7 mM) and PGC-1alpha mRNA content was less (P < 0.05) increased (approximately threefold) than after exercise at 85% of VO(2peak). Likewise, pyruvate dehydrogenase kinase 4 and hexokinase II mRNA expressions were increased (P < 0.05) only after exercise performed at 85% of VO(2peak) in the trained subjects. Hypoxia-inducible factor 2alpha mRNA only increased (P < 0.05) 3 h into recovery in trained subjects, with no difference between the 70% and 85% of VO(2peak) trial. No change in hypoxia-inducible factor 1alpha, phosphofructokinase, citrate synthase, or lactate dehydrogenase, heart and muscle isoforms, mRNA expressions was detected after any of the exercise trials. CONCLUSIONS: The relative intensity of brief intermittent exercise is of major importance for the exercise-induced increase of several mRNA, including PGC-1alpha.
Authors: A Mancini; D Vitucci; G Labruna; E Imperlini; M B Randers; J F Schmidt; M Hagman; T R Andersen; R Russo; S Orrù; P Krustrup; F Salvatore; P Buono Journal: Eur J Appl Physiol Date: 2017-03-01 Impact factor: 3.078
Authors: Matthew M Robinson; Christopher Bell; Frederick F Peelor; Benjamin F Miller Journal: Am J Physiol Regul Integr Comp Physiol Date: 2011-05-25 Impact factor: 3.619
Authors: Michael E Percival; Brian J Martin; Jenna B Gillen; Lauren E Skelly; Martin J MacInnis; Alex E Green; Mark A Tarnopolsky; Martin J Gibala Journal: J Appl Physiol (1985) Date: 2015-09-17