| Literature DB >> 20139595 |
Akio Tonouchi1, Yuichi Hara, Ryousuke Umehara, Tamotsu Sanuki, Takuma Fukusawa, Kazuo Miyairi.
Abstract
An endo-acting family 3 pectate lyase (PL I) gene from Streptomyces thermocarboxydus was cloned and expressed in Escherichia coli. The deduced PL I sequence had highest similarity to a putative pectate lyase of S. coelicolor. Recombinant PL I had significantly lower specific activity, probably a result of incorrect folding, as indicated by circular dichroism spectra analysis.Entities:
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Year: 2010 PMID: 20139595 DOI: 10.1271/bbb.90693
Source DB: PubMed Journal: Biosci Biotechnol Biochem ISSN: 0916-8451 Impact factor: 2.043