| Literature DB >> 20138259 |
Weixia Chen1, Weiyun Zhang, Wenbin Shen, Kongcheng Wang.
Abstract
The acid polysaccharide fraction (APSF) extracted from the mycelia of cultivated Cordyceps sinensis is water-soluble polysaccharide. In this study we evaluated the modulating effects of APSF on murine macrophage cell line RAW264.7. Phagocytotic assay by neutral red and FITC-dextran internalization showed that APSF stimulated the phagocytosis of macrophages. The nitrite levels in the culture supernatant determined using Griess reagent revealed the elevation of NO production after treatment with APSF. RT-PCR and immunocytochemistry assay indicated that APSF promoted both the mRNA and protein expressions of inducible nitric oxide synthase (iNOS). Furthermore, Western blotting demonstrated that NF-kappaB levels in nucleuses increased after APSF treatment, suggesting that APSF probably stimulated macrophage activities by activating the IkappaB-NF-kappaB pathway. Copyright 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20138259 DOI： 10.1016/j.cellimm.2010.01.001
Source DB: PubMed Journal: Cell Immunol ISSN： 0008-8749 Impact factor: 4.868