| Literature DB >> 20133713 |
Davide Serruto1, Tiziana Spadafina, Laura Ciucchi, Lisa A Lewis, Sanjay Ram, Marta Tontini, Laura Santini, Alessia Biolchi, Kate L Seib, Marzia M Giuliani, John J Donnelly, Francesco Berti, Silvana Savino, Maria Scarselli, Paolo Costantino, J Simon Kroll, Clíona O'Dwyer, Jiazhou Qiu, Andrew G Plaut, Richard Moxon, Rino Rappuoli, Mariagrazia Pizza, Beatrice Aricò.
Abstract
GNA2132 is a Neisseria meningitidis antigen of unknown function, discovered by reverse vaccinology, which has been shown to induce bactericidal antibodies in animal models. Here we show that this antigen induces protective immunity in humans and it is recognized by sera of patients after meningococcal disease. The protein binds heparin in vitro through an Arg-rich region and this property correlates with increased survival of the unencapsulated bacterium in human serum. Furthermore, two proteases, the meningococcal NalP and human lactoferrin, cleave the protein upstream and downstream from the Arg-rich region, respectively. We conclude that GNA2132 is an important protective antigen of N. meningitidis and we propose to rename it, Neisserial Heparin Binding Antigen (NHBA).Entities:
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Year: 2010 PMID： 20133713 PMCID： PMC2840514 DOI： 10.1073/pnas.0915162107
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205