Literature DB >> 2013312

Alpha-haemolysin from E. coli. Purification and self-aggregation properties.

H Ostolaza1, B Bartolomé, J L Serra, F de la Cruz, F M Goñi.   

Abstract

An improved, straightforward purification procedure for E.coli alpha-haemolysin has been developed. The protein exists in the form of large aggregates, held together mainly by hydrophobic forces. In the presence of urea or other chaotropic agents, the size of the aggregates decreases, while the specific activity is increased.

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Year:  1991        PMID： 2013312     DOI： 10.1016/0014-5793(91)80291-a

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  Isolation and characterization of Escherichia coli tolC mutants defective in secreting enzymatically active alpha-hemolysin.

Authors:  H Vakharia; G J German; R Misra
Journal:  J Bacteriol       Date:  2001-12       Impact factor: 3.490

2.  Pleiotropic effects of a mutation in rfaC on Escherichia coli hemolysin.

Authors:  M E Bauer; R A Welch
Journal:  Infect Immun       Date:  1997-06       Impact factor: 3.441

3.  Reversible adsorption and nonreversible insertion of Escherichia coli alpha-hemolysin into lipid bilayers.

Authors:  L Bakás; H Ostolaza; W L Vaz; F M Goñi
Journal:  Biophys J       Date:  1996-10       Impact factor: 4.033

4.  Generation of a recombinant Aggregatibacter actinomycetemcomitans RTX toxin in Escherichia coli.

Authors:  Nataliya Balashova; Alexander Giannakakis; Angela C Brown; Evan Koufos; Roland Benz; Tsutomu Arakawa; Hsin-Yao Tang; Edward T Lally
Journal:  Gene       Date:  2018-06-04       Impact factor: 3.688

5.  Variable carbohydrate modifications to the catalytic chains of the RgpA and RgpB proteases of Porphyromonas gingivalis W50.

Authors:  M A Curtis; A Thickett; J M Slaney; M Rangarajan; J Aduse-Opoku; P Shepherd; N Paramonov; E F Hounsell
Journal:  Infect Immun       Date:  1999-08       Impact factor: 3.441

6.  Cytotoxic activity of Kingella kingae RtxA toxin depends on post-translational acylation of lysine residues and cholesterol binding.

Authors:  Adriana Osickova; Nataliya Balashova; Jiri Masin; Miroslav Sulc; Jana Roderova; Tomas Wald; Angela C Brown; Evan Koufos; En Hyung Chang; Alexander Giannakakis; Edward T Lally; Radim Osicka
Journal:  Emerg Microbes Infect       Date:  2018-11-07       Impact factor: 7.163

7.  Complex Multilevel Control of Hemolysin Production by Uropathogenic Escherichia coli.

Authors:  Nguyen Thi Khanh Nhu; Minh-Duy Phan; Brian M Forde; Ambika M V Murthy; Kate M Peters; Christopher J Day; Jessica Poole; Timothy J Kidd; Rodney A Welch; Michael P Jennings; Glen C Ulett; Matthew J Sweet; Scott A Beatson; Mark A Schembri
Journal:  mBio       Date:  2019-10-01       Impact factor: 7.867

8.  In vivo quantification of the secretion rates of the hemolysin A Type I secretion system.

Authors:  Michael H H Lenders; Tobias Beer; Sander H J Smits; Lutz Schmitt
Journal:  Sci Rep       Date:  2016-09-12       Impact factor: 4.379

9.  Channel Formation by LktA of Mannheimia (Pasteurella) haemolytica in Lipid Bilayer Membranes and Comparison of Channel Properties with Other RTX-Cytolysins.

Authors:  Roland Benz; Claudio Piselli; Andrew A Potter
Journal:  Toxins (Basel)       Date:  2019-10-17       Impact factor: 4.546
  9 in total

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