Literature DB >> 20129970

Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus.

Elena Castellanos1, Alicia Aranaz, Jeroen De Buck.   

Abstract

High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20129970      PMCID： PMC2849625          DOI： 10.1128/JCM.02484-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

1.  Source of scotochromogens.

Authors:  E H RUNYON
Journal:  Am Rev Respir Dis       Date:  1959-08

2.  High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution.

Authors:  Luming Zhou; Lesi Wang; Robert Palais; Robert Pryor; Carl T Wittwer
Journal:  Clin Chem       Date:  2005-10       Impact factor: 8.327

3.  Evaluation of two real-time PCR chemistries for the detection of Chlamydophila pneumoniae in clinical specimens.

Authors:  Stephanie L Mitchell; Sona Budhiraja; Kathleen A Thurman; W Lanier Thacker; Jonas M Winchell
Journal:  Mol Cell Probes       Date:  2009-07-30       Impact factor: 2.365

4.  Genetic diversity of Mycobacterium avium subspecies paratuberculosis isolates from goats detected by pulsed-field gel electrophoresis.

Authors:  L de Juan; A Mateos; L Domínguez; J M Sharp; K Stevenson
Journal:  Vet Microbiol       Date:  2005-04-10       Impact factor: 3.293

5.  Molecular characterization of pigmented and nonpigmented isolates of Mycobacterium avium subsp. paratuberculosis.

Authors:  Karen Stevenson; Valerie M Hughes; Lucía de Juan; Neil F Inglis; Frank Wright; J Michael Sharp
Journal:  J Clin Microbiol       Date:  2002-05       Impact factor: 5.948

6.  Paratuberculosis in wild rabbits (Oryctolagus cuniculus)

Authors:  A Greig; K Stevenson; V Perez; A A Pirie; J M Grant; J M Sharp
Journal:  Vet Rec       Date:  1997-02-08       Impact factor: 2.695

7.  Standardisation of restriction fragment length polymorphism analysis for Mycobacterium avium subspecies paratuberculosis.

Authors:  I Pavlik; A Horvathova; L Dvorska; J Bartl; P Svastova; R du Maine; I Rychlik
Journal:  J Microbiol Methods       Date:  1999-10       Impact factor: 2.363

8.  Identification of two groups of Mycobacterium paratuberculosis strains by restriction endonuclease analysis and DNA hybridization.

Authors:  D M Collins; D M Gabric; G W de Lisle
Journal:  J Clin Microbiol       Date:  1990-07       Impact factor: 5.948

9.  Characterization of ovine strains of Mycobacterium paratuberculosis by restriction endonuclease analysis and DNA hybridization.

Authors:  G W De Lisle; D M Collins; H F Huchzermeyer
Journal:  Onderstepoort J Vet Res       Date:  1992-06       Impact factor: 1.792

10.  Paratuberculosis in farmed deer: case reports and DNA characterization of isolates of Mycobacterium paratuberculosis.

Authors:  G W de Lisle; G F Yates; D M Collins
Journal:  J Vet Diagn Invest       Date:  1993-10       Impact factor: 1.279
View more
  3 in total

1.  Multiplexed typing of Mycobacterium avium subsp. paratuberculosis types I, II, and III by Luminex xMAP suspension array.

Authors:  Michele Gastaldelli; Elisabetta Stefani; Antonia Anna Lettini; Nicola Pozzato
Journal:  J Clin Microbiol       Date:  2010-11-17       Impact factor: 5.948

2.  Genetic screening of Fabry patients with EcoTILLING and HRM technology.

Authors:  Caterina Bono; Domenico Nuzzo; Giuseppe Albeggiani; Carmela Zizzo; Daniele Francofonte; Francesco Iemolo; Enzo Sanzaro; Giovanni Duro
Journal:  BMC Res Notes       Date:  2011-09-06

3.  High-resolution melting analysis for the rapid detection of fluoroquinolone and streptomycin resistance in Mycobacterium tuberculosis.

Authors:  Ann S G Lee; Danny C T Ong; Joshua C L Wong; Gilman K H Siu; Wing-Cheong Yam
Journal:  PLoS One       Date:  2012-02-21       Impact factor: 3.240
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.