Literature DB >> 20129109

Quantification and improvement of error rate during ligase detection reaction.

Harumi Ginya1, Ryouhei Matsushita, Masafumi Yohda.   

Abstract

We estimated the actual error rate during ligase detection reaction (LDR), and confirmed that DNA sequences around 3' ends are greatly influenced on the specificity of LDR. Its specificity is increased about 1000 times by introducing a mismatch base near the 3' ends. Copyright (c) 2009 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

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Year:  2009        PMID: 20129109     DOI: 10.1016/j.jbiosc.2009.07.011

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN: 1347-4421            Impact factor:   2.894


  1 in total

1.  Development of a gLCR-based KRAS mutation detection approach and its comparison with other screening methods.

Authors:  Stefan Jenner; Dieter Techel
Journal:  Tumour Biol       Date:  2015-03-27
  1 in total

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