INTRODUCTION: The combination of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) results in the formation of a precipitate. In a previous study, we demonstrated the formation of 4-chloroaniline (PCA) in the precipitate by using x-ray photon spectroscopy (XPS) and time of flight secondary ion mass spectrometry (TOF-SIMS). The TOF-SIMS results showed a peak at 127 amu, which is characteristic of 4-chloroaniline. However, this could also be characteristic of other isomers of 4-chloroaniline such as 2-chloroaniline and 3-chloroaniline. AIMS AND METHODS: The aim of this study was to further identify the precipitate by using gas chromatography-mass spectrometry (GC-MS). RESULTS: The results showed an absence of other aniline derivatives in the precipitate. Only PCA was found. CONCLUSIONS: Further investigations of the precipitate should address the bioavailability of PCA leaching out from dentin and its cytotoxicity. Until the precipitate is studied further, it would appear prudent to minimize its formation by avoiding the use of CHX together with NaOCl. Copyright 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
INTRODUCTION: The combination of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) results in the formation of a precipitate. In a previous study, we demonstrated the formation of 4-chloroaniline (PCA) in the precipitate by using x-ray photon spectroscopy (XPS) and time of flight secondary ion mass spectrometry (TOF-SIMS). The TOF-SIMS results showed a peak at 127 amu, which is characteristic of 4-chloroaniline. However, this could also be characteristic of other isomers of 4-chloroaniline such as 2-chloroaniline and 3-chloroaniline. AIMS AND METHODS: The aim of this study was to further identify the precipitate by using gas chromatography-mass spectrometry (GC-MS). RESULTS: The results showed an absence of other aniline derivatives in the precipitate. Only PCA was found. CONCLUSIONS: Further investigations of the precipitate should address the bioavailability of PCA leaching out from dentin and its cytotoxicity. Until the precipitate is studied further, it would appear prudent to minimize its formation by avoiding the use of CHX together with NaOCl. Copyright 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Authors: Brian Minavi; Adam Youssefi; Ryan Quock; Ariadne Letra; Renato Silva; Timothy C Kirkpatrick; Gena Tribble; Ransome van der Hoeven Journal: Clin Exp Dent Res Date: 2020-12-08
Authors: Colin Eliot; John F Hatton; Gregory P Stewart; Charles F Hildebolt; M Jane Gillespie; James L Gutmann Journal: Odontology Date: 2013-01-19 Impact factor: 2.634